dc.contributor.author | Chen, Bingkun K. | |
dc.contributor.author | Madigan, Nicolas N. | |
dc.contributor.author | Hakim, Jeffrey S. | |
dc.contributor.author | Dadsetan, Mahrokh | |
dc.contributor.author | McMahon, Siobhan S. | |
dc.contributor.author | Yaszemski, Michael J. | |
dc.contributor.author | Windebank, Anthony J. | |
dc.date.accessioned | 2019-08-19T14:24:37Z | |
dc.date.available | 2019-08-19T14:24:37Z | |
dc.date.issued | 2017-03-10 | |
dc.identifier.citation | Chen, Bingkun K., Madigan, Nicolas N., Hakim, Jeffrey S., Dadsetan, Mahrokh, McMahon, Siobhan S., Yaszemski, Michael J., & Windebank, Anthony J. (2018). GDNF Schwann cells in hydrogel scaffolds promote regional axon regeneration, remyelination and functional improvement after spinal cord transection in rats. Journal of Tissue Engineering and Regenerative Medicine, 12(1), e398-e407. doi: 10.1002/term.2431 | en_IE |
dc.identifier.issn | 1932-7005 | |
dc.identifier.uri | http://hdl.handle.net/10379/15321 | |
dc.description.abstract | Positively-charged oligo[poly(ethylene glycol)fumarate] (OPF+) is a biodegradable hydrogel used for spinal cord injury repair. We compared scaffolds containing primary Schwann cells (SCs) to scaffolds delivering SCs genetically modified to secrete high concentrations of glial cell-derived neurotrophic factor (GDNF). Multichannel OPF+ scaffolds loaded with SCs or GDNF-SCs were implanted into transected rat spinal cords for 4 weeks. GDNF-SCs promoted regeneration of more axons into OPF+ scaffolds (2773.0 +/- 396.0) than primary SC OPF+ scaffolds (1666.0 +/- 352.2) (p = 0.0491). This increase was most significant in central and ventral-midline channels of the scaffold. Axonal remyelination was quantitated by stereologic analysis. Increased myelination of regenerating axons was observed in the GDNF-SC group. Myelinating cell and axon complexes were formed by host SCs and not by implanted cells or host oligodendrocytes. Fast Blue retrograde tracing studies determined the rostral-caudal directionality of axonal growth. The number of neurons that projected axons rostrally through the GDNF-SC scaffolds was higher (7929 +/- 1670) than in animals with SC OPF+ scaffolds (1069 +/- 241.5) (p | en_IE |
dc.description.sponsorship | This work was supported by the National Institutes of Health (EB002390) (A. J. W.), the Wilson, Morton and Mayo Foundations (A. J. W.) and the Health Research Board of Ireland (RP/2007/143) (N. M. and S. S. M.). We thank Thomas Ritter, National University of Ireland, Galway, for supplying source plasmids, James Tarara at Mayo Clinic for expertise in confocal microscopy, and Andrew Knight and Trent Chiang for the GDNF ELISA studies. We thank Ann Schmeichel and Shuya Zhang for expertise in immunohistochemistry, Jarred Nesbitt for expert animal care and Jane Meyer for her role in manuscript preparation. | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | Wiley | en_IE |
dc.relation.ispartof | Journal Of Tissue Engineering And Regenerative Medicine | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | scaffolds | en_IE |
dc.subject | spinal cord injury | en_IE |
dc.subject | oligo[poly(ethylene glycol)fumarate] | en_IE |
dc.subject | hydrogel | en_IE |
dc.subject | Schwann cells | en_IE |
dc.subject | glial-derived neurotrophic factor | en_IE |
dc.subject | NEUROTROPHIC FACTOR | en_IE |
dc.subject | POLYMER SCAFFOLDS | en_IE |
dc.subject | CAUDAL STUMPS | en_IE |
dc.subject | INJURY | en_IE |
dc.subject | MYELINATION | en_IE |
dc.subject | GROWTH | en_IE |
dc.subject | GENE | en_IE |
dc.subject | EXPRESSION | en_IE |
dc.subject | PLASTICITY | en_IE |
dc.subject | LOCOMOTION | en_IE |
dc.title | GDNF Schwann cells in hydrogel scaffolds promote regional axon regeneration, remyelination and functional improvement after spinal cord transection in rats | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2019-07-25T11:47:55Z | |
dc.identifier.doi | 10.1002/term.2431 | |
dc.local.publishedsource | https://doi.org/10.1002/term.2431 | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | National Institutes of Health | en_IE |
dc.contributor.funder | Wilson, Morton and Mayo Foundations | en_IE |
dc.internal.rssid | 13909647 | |
dc.local.contact | Siobhan Mcmahon, Department Of Anatomy, Nui, Galway. 2838 Email: siobhan.mcmahon@nuigalway.ie | |
dc.local.copyrightchecked | Yes | |
dc.local.version | ACCEPTED | |
nui.item.downloads | 337 | |