Show simple item record

dc.contributor.authorChen, Bingkun K.
dc.contributor.authorMadigan, Nicolas N.
dc.contributor.authorHakim, Jeffrey S.
dc.contributor.authorDadsetan, Mahrokh
dc.contributor.authorMcMahon, Siobhan S.
dc.contributor.authorYaszemski, Michael J.
dc.contributor.authorWindebank, Anthony J.
dc.date.accessioned2019-08-19T14:24:37Z
dc.date.available2019-08-19T14:24:37Z
dc.date.issued2017-03-10
dc.identifier.citationChen, Bingkun K., Madigan, Nicolas N., Hakim, Jeffrey S., Dadsetan, Mahrokh, McMahon, Siobhan S., Yaszemski, Michael J., & Windebank, Anthony J. (2018). GDNF Schwann cells in hydrogel scaffolds promote regional axon regeneration, remyelination and functional improvement after spinal cord transection in rats. Journal of Tissue Engineering and Regenerative Medicine, 12(1), e398-e407. doi: 10.1002/term.2431en_IE
dc.identifier.issn1932-7005
dc.identifier.urihttp://hdl.handle.net/10379/15321
dc.description.abstractPositively-charged oligo[poly(ethylene glycol)fumarate] (OPF+) is a biodegradable hydrogel used for spinal cord injury repair. We compared scaffolds containing primary Schwann cells (SCs) to scaffolds delivering SCs genetically modified to secrete high concentrations of glial cell-derived neurotrophic factor (GDNF). Multichannel OPF+ scaffolds loaded with SCs or GDNF-SCs were implanted into transected rat spinal cords for 4 weeks. GDNF-SCs promoted regeneration of more axons into OPF+ scaffolds (2773.0 +/- 396.0) than primary SC OPF+ scaffolds (1666.0 +/- 352.2) (p = 0.0491). This increase was most significant in central and ventral-midline channels of the scaffold. Axonal remyelination was quantitated by stereologic analysis. Increased myelination of regenerating axons was observed in the GDNF-SC group. Myelinating cell and axon complexes were formed by host SCs and not by implanted cells or host oligodendrocytes. Fast Blue retrograde tracing studies determined the rostral-caudal directionality of axonal growth. The number of neurons that projected axons rostrally through the GDNF-SC scaffolds was higher (7929 +/- 1670) than in animals with SC OPF+ scaffolds (1069 +/- 241.5) (pen_IE
dc.description.sponsorshipThis work was supported by the National Institutes of Health (EB002390) (A. J. W.), the Wilson, Morton and Mayo Foundations (A. J. W.) and the Health Research Board of Ireland (RP/2007/143) (N. M. and S. S. M.). We thank Thomas Ritter, National University of Ireland, Galway, for supplying source plasmids, James Tarara at Mayo Clinic for expertise in confocal microscopy, and Andrew Knight and Trent Chiang for the GDNF ELISA studies. We thank Ann Schmeichel and Shuya Zhang for expertise in immunohistochemistry, Jarred Nesbitt for expert animal care and Jane Meyer for her role in manuscript preparation.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherWileyen_IE
dc.relation.ispartofJournal Of Tissue Engineering And Regenerative Medicineen
dc.subjectscaffoldsen_IE
dc.subjectspinal cord injuryen_IE
dc.subjectoligo[poly(ethylene glycol)fumarate]en_IE
dc.subjecthydrogelen_IE
dc.subjectSchwann cellsen_IE
dc.subjectglial-derived neurotrophic factoren_IE
dc.subjectNEUROTROPHIC FACTORen_IE
dc.subjectPOLYMER SCAFFOLDSen_IE
dc.subjectCAUDAL STUMPSen_IE
dc.subjectINJURYen_IE
dc.subjectMYELINATIONen_IE
dc.subjectGROWTHen_IE
dc.subjectGENEen_IE
dc.subjectEXPRESSIONen_IE
dc.subjectPLASTICITYen_IE
dc.subjectLOCOMOTIONen_IE
dc.titleGDNF Schwann cells in hydrogel scaffolds promote regional axon regeneration, remyelination and functional improvement after spinal cord transection in ratsen_IE
dc.typeArticleen_IE
dc.date.updated2019-07-25T11:47:55Z
dc.identifier.doi10.1002/term.2431
dc.local.publishedsourcehttps://doi.org/10.1002/term.2431en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderNational Institutes of Healthen_IE
dc.contributor.funderWilson, Morton and Mayo Foundationsen_IE
dc.internal.rssid13909647
dc.local.contactSiobhan Mcmahon, Department Of Anatomy, Nui, Galway. 2838 Email: siobhan.mcmahon@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
nui.item.downloads102


Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:

Thumbnail

This item appears in the following Collection(s)

Show simple item record