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dc.contributor.authorAbu-Rub, Mohammad T.
dc.contributor.authorNewland, Ben
dc.contributor.authorNaughton, Michelle
dc.contributor.authorWang, Wenxin
dc.contributor.authorMcMahon, Siobhan
dc.contributor.authorPandit, Abhay
dc.date.accessioned2019-08-19T14:09:57Z
dc.date.available2019-08-19T14:09:57Z
dc.date.issued2016-10-13
dc.identifier.citationAbu-Rub, Mohammad T., Newland, Ben, Naughton, Michelle, Wang, Wenxin, McMahon, Siobhan, & Pandit, Abhay. (2016). Non-viral xylosyltransferase-1 siRNA delivery as an effective alternative to chondroitinase in an in vitro model of reactive astrocytes. Neuroscience, 339, 267-275. doi: https://doi.org/10.1016/j.neuroscience.2016.10.026en_IE
dc.identifier.issn1873-7544
dc.identifier.urihttp://hdl.handle.net/10379/15320
dc.description.abstractReactive astrocytosis and the subsequent glial scar is ubiquitous to injuries of the central nervous system, especially spinal cord injury (SCI) and primarily serves to protect against further damage, but is also a prominent inhibitor of regeneration. Manipulating the glial scar by targeting chondroitin sulfate proteoglycans (CSPGs) has been the focus of much study as a means to improve axon regeneration and subsequently functional recovery. In this study we investigate the ability of small interfering RNA (siRNA) delivered by a non-viral polymer vector to silence the rate limiting enzyme involved in CSPG synthesis. Gene expression of this enzyme, xylosyltransferase-1, was silenced by 65% in Neu7 astrocytes which conferred a reduced expression of CSPGs. Furthermore, conditioned medium taken from treated Neu7s, or co-culture experiments with dorsal root ganglia (DRG) showed that siRNA treatment resulted in a more permissive environment for DRG neurite outgrowth than treatment with chondroitinase ABC alone. These results indicate that there is a role for targeted siRNA therapy using polymeric vectors to facilitate regeneration of injured axons following central nervous system injury. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.en_IE
dc.description.sponsorshipThe authors would like to thank Science Foundation Ireland for financial assistance under Grant no. 07/SRC/B1163.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofNeuroscienceen
dc.subjectpolymeren_IE
dc.subjecttransfection agenten_IE
dc.subjectsiRNAen_IE
dc.subjectCSPGen_IE
dc.subjectneurite outgrowthen_IE
dc.subjectSPINAL-CORD-INJURYen_IE
dc.subjectRESPIRATORY SYNCYTIAL VIRUSen_IE
dc.subjectRNA INTERFERENCE THERAPYen_IE
dc.subjectSINGLE CYCLIZED MOLECULEen_IE
dc.subjectCENTRAL-NERVOUS-SYSTEMen_IE
dc.subjectMULTI-VINYL MONOMERSen_IE
dc.subjectSULFATE PROTEOGLYCANSen_IE
dc.subjectNEURITE OUTGROWTHen_IE
dc.subjectAXON GROWTHen_IE
dc.subjectGENE DELIVERYen_IE
dc.titleNon-viral xylosyltransferase-1 siRNA delivery as an effective alternative to chondroitinase in an in vitro model of reactive astrocytesen_IE
dc.typeArticleen_IE
dc.date.updated2019-07-25T11:50:03Z
dc.identifier.doi10.1016/j.neuroscience.2016.10.026
dc.local.publishedsourcehttps://doi.org/10.1016/j.neuroscience.2016.10.026en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.internal.rssid11876657
dc.local.contactSiobhan Mcmahon, Department Of Anatomy, Nui, Galway. 2838 Email: siobhan.mcmahon@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Strategic Research Cluster/07/SRC/B1163/IE/SRC NFB: Network of Excellence for Functional Biomaterials (NFB)/en_IE
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