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dc.contributor.authorPatar, Azim
dc.contributor.authorDockery, Peter
dc.contributor.authorHoward, Linda
dc.contributor.authorMcMahon, Siobhan
dc.date.accessioned2019-07-23T07:47:45Z
dc.date.issued2018-09-26
dc.identifier.citationPatar, Azim, Dockery, Peter, Howard, Linda, & McMahon, Siobhan. (2019). Analysis of reactive astrocytes and NG2 proteoglycan in ex vivo rat models of spinal cord injury. Journal of Neuroscience Methods, 311, 418-425. doi: https://doi.org/10.1016/j.jneumeth.2018.09.027en_IE
dc.identifier.issn1872-678X
dc.identifier.urihttp://hdl.handle.net/10379/15295
dc.description.abstractBackground: The use of animals to model spinal cord injury (SCI) requires extensive post-operative care and can be expensive, which makes an altemative model extremely attractive. The use ofex vivo slice cultures is an alternative way to study the pathophysiological changes that can mimic in vivo conditions and support the 3Rs (replacement, reduction and refinement) of animal use in SCI research models.New method: In this study the presence of reactive astrocytes and NG2 proteoglycans was investigated in two ex vivo models of SCI; stab injury and transection injury. Stereological analysis to measure immunohistochemical staining was performed on the scar and injury zones to detect astrocytes and the chondroitin sulphate proteoglycan NG2.Results: The volume fraction (Vv) of reactive astrocytes and NG2 proteoglycans increased significantly between day 3 and day 10 post injury in both ex vivo models. This data shows how ex vivo SCI models are a useful research tool allowing reduction of research cost and time involved in carrying out animal studies, as well as reducing the numbers of animals used.Comparison with existing method: This is the first evidence of an ex vivo stab injury model of SCI and also the first comparison of immunohistochemical staining for injury markers within stab injured and transection injured ex vivo slice cultures.Conclusions: The use of organotypic slice culture models provide a simple way to study the cellular consequences following SCI and they can also be used as a platform for potential therapeutics regimes for the treatment of SCI.en_IE
dc.description.sponsorshipThe authors acknowledge the facilities, scientific and technical assistance (Mr Mark Canney and Dr Kerry Thompson) of the Centre for Microscopy and Imaging at the National University of Ireland, Galway (www.imaging.nuigalway.ie), a facility which is co-funded by the Irish Government’s Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007–2013. Funding for this project was provided by the Malaysia Ministry of Education and the College of Medicine, Nursing and Health Science at NUI Galway, Ireland.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofJournal Of Neuroscience Methodsen
dc.subjectEx vivo slice cultureen_IE
dc.subjectSpinal cord injuryen_IE
dc.subjectStab injuryen_IE
dc.subjectTransection injuryen_IE
dc.subjectReactive astrocytesen_IE
dc.subjectNG2 proteoglycansen_IE
dc.subjectORGANOTYPIC SLICE CULTURESen_IE
dc.subjectREGENERATIONen_IE
dc.subjectPLATFORMen_IE
dc.subjectCELLSen_IE
dc.titleAnalysis of reactive astrocytes and NG2 proteoglycan in ex vivo rat models of spinal cord injuryen_IE
dc.typeArticleen_IE
dc.date.updated2019-07-23T07:17:56Z
dc.identifier.doi10.1016/j.jneumeth.2018.09.027
dc.local.publishedsourcehttps://doi.org/10.1016/j.jneumeth.2018.09.027en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderMinistry of Higher Education, Malaysiaen_IE
dc.contributor.funderCollege of Medicine, Nursing and Health Sciences, National University of Ireland Galwayen_IE
dc.description.embargo2020-03-26
dc.internal.rssid15594560
dc.local.contactSiobhan Mcmahon, Department Of Anatomy, Nui, Galway. 2838 Email: siobhan.mcmahon@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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