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dc.contributor.authorRennie, Martin L.
dc.contributor.authorCrowley, Peter B.
dc.date.accessioned2019-06-20T08:17:45Z
dc.date.issued2019-03-12
dc.identifier.citationRennie, Martin L., & Crowley, Peter B. (2019). A Thermodynamic Model of Auto-regulated Protein Assembly by a Supramolecular Scaffold. ChemPhysChem, 20(8), 1011-1017. doi: 10.1002/cphc.201900153en_IE
dc.identifier.issn1439-4235
dc.identifier.issn1439-7641
dc.identifier.urihttp://hdl.handle.net/10379/15243
dc.description.abstractLigand-mediated regulation of protein assembly occurs frequently in different cellular contexts. Auto-regulated assembly, where a ligand acts as its own competitive inhibitor, provides a mechanism for exquisite control of assembly. Unlike simple protein-ligand systems a quantification of the binding thermodynamics is not straightforward. Here, we characterize the interactions of a recently identified model system in which the oligomerization of cytochrome c is controlled by sulfonato-calix[8]arene, an anionic supramolecular scaffold. Isothermal titration calorimetry and thermodynamic modelling, in combination with Bayesian fitting, were used to quantify the ligand binding and assembly equilibria for this system. The approach and variations of this model may prove useful for the analysis of auto-regulated protein assembly in general.en_IE
dc.description.sponsorshipThis research was supported by NUI Galway and Science Foundation Ireland (grants 13/ERC/B2912 and 13/CDA/2168 to PBC).en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherWileyen_IE
dc.relation.ispartofChemphyschemen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectISOTHERMAL TITRATION CALORIMETRYen_IE
dc.subjectLIGAND-BINDINGen_IE
dc.subjectDIMERIZATIONen_IE
dc.subjectPHYTATEen_IE
dc.subjectCOOPERATIVITYen_IE
dc.subjectSOLUBILITYen_IE
dc.subjectMECHANISMen_IE
dc.titleA thermodynamic model of auto-regulated protein assembly by a supramolecular scaffolden_IE
dc.typeArticleen_IE
dc.date.updated2019-06-20T07:35:16Z
dc.identifier.doi10.1002/cphc.201900153
dc.local.publishedsourcehttps://doi.org/10.1002/cphc.201900153en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderNational University of Ireland, Galwayen_IE
dc.contributor.funderScience Foundation Irelanden_IE
dc.description.embargo2020-03-12
dc.internal.rssid16261455
dc.local.contactPeter Bernard Crowley, Chemistry, Room 115, Arts/Science Building, Nui Galway. 2480 Email: peter.crowley@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI ERC Development Programme/13/ERC/B2912/IE/Supramolecular Approaches to Protein Surface Recognition and Assembly (SupraSurf)/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Career Development Award/13/CDA/2168/IE/Supramolecular Approaches to Protein Surface Recognition and Self Assembly/en_IE
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland