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dc.contributor.authorHamon, Siobhan M.
dc.contributor.authorGriffin, Tomás P.
dc.contributor.authorIslam, Md Nahidul
dc.contributor.authorWall, Deirdre
dc.contributor.authorGriffin, Matthew D.
dc.contributor.authorO’Shea, Paula M.
dc.date.accessioned2019-03-13T11:37:20Z
dc.date.issued2018-09-15
dc.identifier.citationHamon Siobhan, M., Griffin Tomás, P., Islam Md, Nahidul, Wall, Deirdre, Griffin Matthew, D., & O’Shea Paula, M. (2019). Defining reference intervals for a serum growth differentiation factor-15 (GDF-15) assay in a Caucasian population and its potential utility in diabetic kidney disease (DKD). Clinical Chemistry and Laboratory Medicine (CCLM), 57(4), 510. doi: 10.1515/cclm-2018-0534en_IE
dc.identifier.issn1437-4331
dc.identifier.urihttp://hdl.handle.net/10379/15017
dc.description.abstractBACKGROUND: Growth differentiation factor-15 (GDF-15), a stress responsive cytokine, is a promising biomarker of renal functional decline in diabetic kidney disease (DKD). This study aimed primarily to establish normative data and secondarily to evaluate the potential utility of GDF-15 in DKD using Roche Diagnostics electrochemiluminescence immunoassay (ECLIA) in an Irish Caucasian population. METHODS: Following informed consent, 188 healthy volunteers and 128 participants with diabetes (72 with and 56 without DKD) were recruited to a cross-sectional study. Baseline demographics, anthropometric measurements and laboratory measurements were recorded. Blood for GDF-15 measurement was collected into plain specimen tubes kept at room temperature and processed (centrifugation, separation of serum, freezing at -80 °C) within 1 h of phlebotomy pending batch analyses. Reference intervals were determined using the 2.5th and 97.5th percentiles for serum GDF-15 concentration. RESULTS: Of 188 healthy participants, 63 failed to meet study inclusion criteria. The reference interval for serum GDF-15 was 399 ng/L (90% confidence interval [CI]: 399-399) - 1335 ng/L (90% CI: 1152-1445). Receiver operator characteristics (ROC) curve analysis for DKD determined the area under the ROC curve to be 0.931 (95% CI: 0.893-0.959; p1136 ng/L providing a diagnostic sensitivity and specificity of 94.4% and 79%, respectively, and positive likelihood ratio of 4.5:1 (95% CI: 3.4-6.0). CONCLUSIONS: The reference interval for serum GDF-15 in a healthy Irish Caucasian population using Roche Diagnostics ECLIA was established and a preliminary determination of the potential of GDF-15 as a screening test for DKD was made. Further prospective validation with a larger DKD cohort will be required before the cutoff presented here is recommended for clinical use.en_IE
dc.description.sponsorshipWe wish to express our gratitude to all volunteers and patients who made this study possible. Special thanks to the scientific, nursing and medical staff at the Centre for Endocrinology, Diabetes and Metabolism, and the Department of Clinical Biochemistry at Saolta University Health Care Group (SUHCG), Galway University Hospitals.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherDe Gruyteren_IE
dc.relation.ispartofClin Chem Lab Meden
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectdiabetes mellitus (DM)en_IE
dc.subjectdiabetic kidney disease (DKD)en_IE
dc.subjectelectrochemiluminescenceen_IE
dc.subjectgrowth differentiation factor-15 (GDF-15)en_IE
dc.subjectimmunoassayen_IE
dc.subjectreference intervalen_IE
dc.titleDefining reference intervals for a serum growth differentiation factor-15 (GDF-15) assay in a Caucasian population and its potential utility in diabetic kidney disease (DKD).en_IE
dc.typeArticleen_IE
dc.date.updated2019-03-13T10:57:00Z
dc.identifier.doi10.1515/cclm-2018-0534
dc.local.publishedsourcehttps://doi.org/10.1515/cclm-2018-0534en_IE
dc.description.peer-reviewedpeer-reviewed
dc.description.embargo2019-09-15
dc.internal.rssid16006772
dc.local.contactMatthew Dallas Griffin, Remedi, Biomedical Sciences Buil, Corrib Village, Dangan, Nui Galway. 5436 Email: matthew.griffin@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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