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dc.contributor.authorYao, L
dc.contributor.authorDaly, W
dc.contributor.authorNewland, B
dc.contributor.authorYao, S
dc.contributor.authorWang, W
dc.contributor.authorChen, B K K
dc.contributor.authorMadigan, N
dc.contributor.authorWindebank, A
dc.contributor.authorPandit, A
dc.date.accessioned2018-09-20T16:28:48Z
dc.date.available2018-09-20T16:28:48Z
dc.date.issued2013-07-25
dc.identifier.citationYao, L; Daly, W; Newland, B; Yao, S; Wang, W; Chen, B K K; Madigan, N; Windebank, A; Pandit, A (2013). Improved axonal regeneration of transected spinal cord mediated by multichannel collagen conduits functionalized with neurotrophin-3 gene. Gene Therapy 20 (12), 1149-1157
dc.identifier.issn0969-7128,1476-5462
dc.identifier.urihttp://hdl.handle.net/10379/14488
dc.description.abstractFunctionalized biomaterial scaffolds targeted at improving axonal regeneration by enhancing guided axonal growth provide a promising approach for the repair of spinal cord injury. Collagen neural conduits provide structural guidance for neural tissue regeneration, and in this study it is shown that these conduits can also act as a reservoir for sustained gene delivery. Either a G-luciferase marker gene or a neurotrophin-3-encoding gene, complexed to a non-viral, cyclized, PEGylated transfection vector, was loaded within a multichannel collagen conduit. The complexed genes were then released in a controlled fashion using a dual release system both in vitro and in vivo. For evaluation of their biological performance, the loaded conduits were implanted into the completely transected rat thoracic spinal cord (T8-T10). Aligned axon regeneration through the channels of conduits was observed one month post-surgery. The conduits delivering neurotrophin-3 polyplexes resulted in significantly increased neurotrophin-3 levels in the surrounding tissue and a statistically higher number of regenerated axons versus the control conduits (P<0.05). This study suggests that collagen neural conduits delivering a highly effective non-viral therapeutic gene may hold promise for repair of the injured spinal cord.
dc.publisherSpringer Nature
dc.relation.ispartofGene Therapy
dc.subjectnon-viral gene delivery
dc.subjectneural conduits
dc.subject2-(dimethylamino)ethyl methacrylate
dc.subjectspinal cord injury
dc.subjectperipheral-nerve regeneration
dc.subjectneural stem-cells
dc.subjectcorticospinal tract
dc.subjectschwann-cells
dc.subjectadult-rats
dc.subjecttransgene expression
dc.subjectpolymer scaffolds
dc.subjectknot polymer
dc.subjectin-vivo
dc.subjectrecovery
dc.titleImproved axonal regeneration of transected spinal cord mediated by multichannel collagen conduits functionalized with neurotrophin-3 gene
dc.typeArticle
dc.identifier.doi10.1038/gt.2013.42
dc.local.publishedsourcehttps://www.nature.com/articles/gt201342.pdf
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