dc.contributor.author | Stephan, A. K. | |
dc.contributor.author | Kliszczak, M. | |
dc.contributor.author | Dodson, H. | |
dc.contributor.author | Cooley, C. | |
dc.contributor.author | Morrison, C. G. | |
dc.date.accessioned | 2018-09-20T16:25:30Z | |
dc.date.available | 2018-09-20T16:25:30Z | |
dc.date.issued | 2011-01-18 | |
dc.identifier.citation | Stephan, A. K. Kliszczak, M.; Dodson, H.; Cooley, C.; Morrison, C. G. (2011). Roles of vertebrate smc5 in sister chromatid cohesion and homologous recombinational repair. Molecular and Cellular Biology 31 (7), 1369-1381 | |
dc.identifier.issn | 0270-7306 | |
dc.identifier.uri | http://hdl.handle.net/10379/14011 | |
dc.description.abstract | The structural maintenance of chromosomes (Smc) family members Smc5 and Smc6 are both essential in budding and fission yeasts. Yeast smc5/6 mutants are hypersensitive to DNA damage, and Smc5/6 is recruited to HO-induced double-strand breaks (DSBs), facilitating intersister chromatid recombinational repair. To determine the role of the vertebrate Smc5/6 complex during the normal cell cycle, we generated an Smc5-deficient chicken DT40 cell line using gene targeting. Surprisingly, Smc5(-) cells were viable, although they proliferated more slowly than controls and showed mitotic abnormalities. Smc5-deficient cells were sensitive to methyl methanesulfonate and ionizing radiation (IR) and showed increased chromosome aberration levels upon irradiation. Formation and resolution of Rad51 and gamma-H2AX foci after irradiation were altered in Smc5 mutants, suggesting defects in homologous recombinational (HR) repair of DNA damage. Ku70(-/-) Smc5(-) cells were more sensitive to IR than either single mutant, with Rad54(-/-) Smc5(-) cells being no more sensitive than Rad54(-/-) cells, consistent with an HR function for the vertebrate Smc5/6 complex. Although gene targeting occurred at wild-type levels, recombinational repair of induced double-strand breaks was reduced in Smc5(-) cells. Smc5 loss increased sister chromatid exchanges and sister chromatid separation distances in mitotic chromosomes. We conclude that Smc5/6 regulates recombinational repair by ensuring appropriate sister chromatid cohesion. | |
dc.publisher | American Society for Microbiology | |
dc.relation.ispartof | Molecular and Cellular Biology | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | double-strand breaks | |
dc.subject | DNA-damage responses | |
dc.subject | smc5-smc6 complex | |
dc.subject | fission yeast | |
dc.subject | saccharomyces-cerevisiae | |
dc.subject | mammalian-cells | |
dc.subject | sumo ligase | |
dc.subject | checkpoint responses | |
dc.subject | replication forks | |
dc.subject | protein complex | |
dc.title | Roles of vertebrate smc5 in sister chromatid cohesion and homologous recombinational repair | |
dc.type | Article | |
dc.identifier.doi | 10.1128/mcb.00786-10 | |
dc.local.publishedsource | http://mcb.asm.org/content/31/7/1369.full.pdf | |
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