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dc.contributor.authorSong, Boya
dc.contributor.authorShen, Min
dc.contributor.authorJiang, Di
dc.contributor.authorMalla, Spundana
dc.contributor.authorMosa, Islam M.
dc.contributor.authorChoudhary, Dharamainder
dc.contributor.authorRusling, James F.
dc.date.accessioned2018-09-20T16:25:15Z
dc.date.available2018-09-20T16:25:15Z
dc.date.issued2016-01-01
dc.identifier.citationSong, Boya; Shen, Min; Jiang, Di; Malla, Spundana; Mosa, Islam M. Choudhary, Dharamainder; Rusling, James F. (2016). Microfluidic array for simultaneous detection of dna oxidation and dna-adduct damage. The Analyst 141 (20), 5722-5729
dc.identifier.issn0003-2654,1364-5528
dc.identifier.urihttp://hdl.handle.net/10379/13983
dc.description.abstractExposure to chemical pollutants and pharmaceuticals may cause health issues caused by metabolite-related toxicity. This paper reports a new microfluidic electrochemical sensor array with the ability to simultaneously detect common types of DNA damage including oxidation and nucleobase adduct formation. Sensors in the 8-electrode screen-printed carbon array were coated with thin films of metallopolymers osmium or ruthenium bipyridyl-poly( vinylpyridine) chloride (OsPVP, RuPVP) along with DNA and metabolic enzymes by layer-by-layer electrostatic assembly. After a reaction step in which test chemicals and other necessary reagents flow over the array, OsPVP selectively detects oxidized guanines on the DNA strands, and RuPVP detects DNA adduction by metabolites on nucleobases. We demonstrate array performance for test chemicals including 17 beta-estradiol (E-2), its metabolites 4-hydroxyestradiol (4-OHE2), 2-hydroxyestradiol (2-OHE2), catechol, 2-nitrosotoluene (2-NO-T), 4-(methylnitrosamino)-1-(3-pyridyl)1-butanone (NNK), and 2-acetylaminofluorene (2-AAF). Results revealed DNA-adduct and oxidation damage in a single run to provide a metabolic-genotoxic chemistry screen. The array measures damage directly in unhydrolyzed DNA, and is less expensive, faster, and simpler than conventional methods to detect DNA damage. The detection limit for oxidation is 672 8-oxodG per 10(6) bases. Each sensor requires only 22 ng of DNA, so the mass detection limit is 15 pg (similar to 10 pmol) 8-oxodG.
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.ispartofThe Analyst
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjecttandem mass-spectrometry
dc.subjectliquid-chromatography
dc.subjectdirect electrochemiluminescence
dc.subjectreactive metabolites
dc.subjectultrathin films
dc.subjectbreast-cancer
dc.subjectin-vitro
dc.subjectcarcinogenesis
dc.subjectmutations
dc.subjecttoxicity
dc.titleMicrofluidic array for simultaneous detection of dna oxidation and dna-adduct damage
dc.typeArticle
dc.identifier.doi10.1039/c6an01237j
dc.local.publishedsourcehttp://europepmc.org/articles/pmc5048564?pdf=render
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland