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dc.contributor.authorSir, Joo-Hee
dc.contributor.authorPütz, Monika
dc.contributor.authorDaly, Owen
dc.contributor.authorMorrison, Ciaran G.
dc.contributor.authorDunning, Mark
dc.contributor.authorKilmartin, John V.
dc.contributor.authorGergely, Fanni
dc.date.accessioned2018-09-20T16:24:54Z
dc.date.available2018-09-20T16:24:54Z
dc.date.issued2013-12-02
dc.identifier.citationSir, Joo-Hee; Pütz, Monika; Daly, Owen; Morrison, Ciaran G. Dunning, Mark; Kilmartin, John V.; Gergely, Fanni (2013). Loss of centrioles causes chromosomal instability in vertebrate somatic cells. The Journal of Cell Biology 203 (5), 747-756
dc.identifier.issn0021-9525,1540-8140
dc.identifier.urihttp://hdl.handle.net/10379/13936
dc.description.abstractMost animal cells contain a centrosome, which comprises a pair of centrioles surrounded by an ordered pericentriolar matrix (PCM). Although the role of this organelle in organizing the mitotic spindle poles is well established, its precise contribution to cell division and cell survival remains a subject of debate. By genetically ablating key components of centriole biogenesis in chicken DT40 B cells, we generated multiple cell lines that lack centrioles. PCM components accumulated in acentriolar microtubule (MT)-organizing centers but failed to adopt a higher-order structure, as shown by three-dimensional structured illumination microscopy. Cells without centrioles exhibited both a delay in bipolar spindle assembly and a high rate of chromosomal instability. Collectively, our results expose a vital role for centrosomes in establishing a mitotic spindle geometry that facilitates correct kinetochore-MT attachments. We propose that centrosomes are essential in organisms in which rapid segregation of a large number of chromosomes needs to be attained with fidelity.
dc.publisherRockefeller University Press
dc.relation.ispartofThe Journal of Cell Biology
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectDNA-damage response
dc.subjectpericentriolar material
dc.subjectmammalian-cells
dc.subjectmis-segregation
dc.subjectcentrosome
dc.subjectduplication
dc.subjectreveals
dc.subjectorganization
dc.subjectattachment
dc.subjectaneuploidy
dc.titleLoss of centrioles causes chromosomal instability in vertebrate somatic cells
dc.typeArticle
dc.identifier.doi10.1083/jcb.201309038
dc.local.publishedsourcehttp://europepmc.org/articles/pmc3857480?pdf=render
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