dc.contributor.author | Siepenkoetter, Till | |
dc.contributor.author | Salaj-Kosla, Urszula | |
dc.contributor.author | Xiao, Xinxin | |
dc.contributor.author | Conghaile, Peter Ó | |
dc.contributor.author | Pita, Marcos | |
dc.contributor.author | Ludwig, Roland | |
dc.contributor.author | Magner, Edmond | |
dc.date.accessioned | 2018-09-20T16:24:40Z | |
dc.date.available | 2018-09-20T16:24:40Z | |
dc.date.issued | 2016-11-25 | |
dc.identifier.citation | Siepenkoetter, Till; Salaj-Kosla, Urszula; Xiao, Xinxin; Conghaile, Peter Ó; Pita, Marcos; Ludwig, Roland; Magner, Edmond (2016). Immobilization of redox enzymes on nanoporous gold electrodes: applications in biofuel cells. ChemPlusChem 82 (4), 553-560 | |
dc.identifier.issn | 2192-6506 | |
dc.identifier.uri | http://hdl.handle.net/10379/13905 | |
dc.description.abstract | Nanoporous gold (NPG) electrodes were prepared by dealloying sputtered gold: silver alloys. Electrodes of different thicknesses and pore sizes areas were prepared by varying the temperature and duration of the dealloying procedure; these were then used as supports for FAD-dependent glucose dehydrogenase (GDH) (Glomorella cingulata) and bilirubin oxidase (BOx) (Myrothecium verrucaria). Glucose dehydrogenase was immobilized by drop-casting a solution of the enzyme with an osmium redox polymer together with a crosslinked polymer, whereas bilirubin oxidase was attached covalently through carbodiimide coupling to a diazonium-modified NPG electrode. The stability of the bilirubin-oxidase-modified NPG electrode was significantly improved in comparison with that of a planar gold electrode. Enzyme fuel cells were also prepared; the optimal response was obtained with a BOx-modified NPG cathode (500 nm thickness) and a GDH-modified anode (300 nm), which generated power densities of 17.5 and 7.0 mu W cm(-2) in phosphate-buffered saline and artificial serum, respectively. | |
dc.publisher | Wiley-Blackwell | |
dc.relation.ispartof | ChemPlusChem | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | biofuel cells | |
dc.subject | electrochemistry | |
dc.subject | gold | |
dc.subject | mesoporous materials | |
dc.subject | nanoelectrodes | |
dc.subject | thermostable glucose-dehydrogenase | |
dc.subject | bilirubin oxidase | |
dc.subject | bioelectrochemical applications | |
dc.subject | electrochemical biosensors | |
dc.subject | cellobiose dehydrogenase | |
dc.subject | myrothecium-verrucaria | |
dc.subject | oxygen reduction | |
dc.subject | diazonium salts | |
dc.subject | recent progress | |
dc.subject | carbon | |
dc.title | Immobilization of redox enzymes on nanoporous gold electrodes: applications in biofuel cells | |
dc.type | Article | |
dc.identifier.doi | 10.1002/cplu.201600455 | |
dc.local.publishedsource | https://digital.csic.es/bitstream/10261/163726/1/accesoRestringido.pdf | |
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