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dc.contributor.authorSiepenkoetter, Till
dc.contributor.authorSalaj-Kosla, Urszula
dc.contributor.authorXiao, Xinxin
dc.contributor.authorConghaile, Peter Ó
dc.contributor.authorPita, Marcos
dc.contributor.authorLudwig, Roland
dc.contributor.authorMagner, Edmond
dc.date.accessioned2018-09-20T16:24:40Z
dc.date.available2018-09-20T16:24:40Z
dc.date.issued2016-11-25
dc.identifier.citationSiepenkoetter, Till; Salaj-Kosla, Urszula; Xiao, Xinxin; Conghaile, Peter Ó; Pita, Marcos; Ludwig, Roland; Magner, Edmond (2016). Immobilization of redox enzymes on nanoporous gold electrodes: applications in biofuel cells. ChemPlusChem 82 (4), 553-560
dc.identifier.issn2192-6506
dc.identifier.urihttp://hdl.handle.net/10379/13905
dc.description.abstractNanoporous gold (NPG) electrodes were prepared by dealloying sputtered gold: silver alloys. Electrodes of different thicknesses and pore sizes areas were prepared by varying the temperature and duration of the dealloying procedure; these were then used as supports for FAD-dependent glucose dehydrogenase (GDH) (Glomorella cingulata) and bilirubin oxidase (BOx) (Myrothecium verrucaria). Glucose dehydrogenase was immobilized by drop-casting a solution of the enzyme with an osmium redox polymer together with a crosslinked polymer, whereas bilirubin oxidase was attached covalently through carbodiimide coupling to a diazonium-modified NPG electrode. The stability of the bilirubin-oxidase-modified NPG electrode was significantly improved in comparison with that of a planar gold electrode. Enzyme fuel cells were also prepared; the optimal response was obtained with a BOx-modified NPG cathode (500 nm thickness) and a GDH-modified anode (300 nm), which generated power densities of 17.5 and 7.0 mu W cm(-2) in phosphate-buffered saline and artificial serum, respectively.
dc.publisherWiley-Blackwell
dc.relation.ispartofChemPlusChem
dc.subjectbiofuel cells
dc.subjectelectrochemistry
dc.subjectgold
dc.subjectmesoporous materials
dc.subjectnanoelectrodes
dc.subjectthermostable glucose-dehydrogenase
dc.subjectbilirubin oxidase
dc.subjectbioelectrochemical applications
dc.subjectelectrochemical biosensors
dc.subjectcellobiose dehydrogenase
dc.subjectmyrothecium-verrucaria
dc.subjectoxygen reduction
dc.subjectdiazonium salts
dc.subjectrecent progress
dc.subjectcarbon
dc.titleImmobilization of redox enzymes on nanoporous gold electrodes: applications in biofuel cells
dc.typeArticle
dc.identifier.doi10.1002/cplu.201600455
dc.local.publishedsourcehttps://digital.csic.es/bitstream/10261/163726/1/accesoRestringido.pdf
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