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dc.contributor.authorSchmaußer, B.
dc.contributor.authorEndrich, S.
dc.contributor.authorBeier, D.
dc.contributor.authorMoran, A. P.
dc.contributor.authorBurek, C. J.
dc.contributor.authorRosenwald, A.
dc.contributor.authorRieckmann, P.
dc.contributor.authorMüller-Hermelink, H.-K.
dc.contributor.authorEck, M.
dc.date.accessioned2018-09-20T16:24:07Z
dc.date.available2018-09-20T16:24:07Z
dc.date.issued2008-03-05
dc.identifier.citationSchmaußer, B. Endrich, S.; Beier, D.; Moran, A. P.; Burek, C. J.; Rosenwald, A.; Rieckmann, P.; Müller-Hermelink, H.-K.; Eck, M. (2008). Triggering receptor expressed on myeloid cells-1 (trem-1) expression on gastric epithelium: implication for a role of trem-1 in helicobacter pylori infection. Clinical & Experimental Immunology 152 (1), 88-94
dc.identifier.issn0009-9104
dc.identifier.urihttp://hdl.handle.net/10379/13828
dc.description.abstractIn Helicobacter pylori gastritis gastric epithelium plays a central role in the innate immunity to H. pylori. However, epithelial receptors interacting with H. pylori have been poorly characterized so far. Recently a new triggering receptor expressed on myeloid cells-1 (TREM-1) has been identified on human neutrophils and monocytes. On these cells TREM-1 triggers innate immunity by stimulating the secretion of interleukin (IL)-8 and tumour necrosis factor (TNF)-alpha and thus amplifies bacterial-induced inflammation. In this study expression and function of TREM-1 in gastric epithelium exposed to H. pylori has been investigated. TREM-1 mRNA and protein were expressed on gastric epithelial cell lines as demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence activated cell sorter analysis. Gastric epithelial TREM-1 expression was up-regulated directly by H. pylori and was independent of epithelial IL-8 induced by H. pylori. Immunohistochemistry and tissue RT-PCR demonstrated significantly stronger TREM-1 expression in H. pylori gastritis compared with the non-inflamed gastric mucosa supporting in vivo that epithelial TREM-1 is up-regulated during H. pylori infection. Stimulation of gastric epithelial TREM-1 receptor resulted in IL-8 up-regulation on mRNA and protein level, as shown by real-time PCR and immunoassay. This is the first study localizing TREM-1 on gastric epithelium. Functional data suggest that TREM-1 expressed on gastric epithelium amplifies inflammation of the underlying gastric mucosa by up-regulation of IL-8.
dc.publisherWiley-Blackwell
dc.relation.ispartofClinical & Experimental Immunology
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectgastric epithelium
dc.subjecth. pylori gastritis
dc.subjecth. pylori
dc.subjectinnate immunity
dc.subjecttrem-1
dc.subjectinflammatory responses
dc.subjectcutting edge
dc.subjectneutrophils
dc.titleTriggering receptor expressed on myeloid cells-1 (trem-1) expression on gastric epithelium: implication for a role of trem-1 in helicobacter pylori infection
dc.typeArticle
dc.identifier.doi10.1111/j.1365-2249.2008.03608.x
dc.local.publishedsourcehttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2008.03608.x/pdf
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