dc.contributor.author | Schmaußer, B. | |
dc.contributor.author | Endrich, S. | |
dc.contributor.author | Beier, D. | |
dc.contributor.author | Moran, A. P. | |
dc.contributor.author | Burek, C. J. | |
dc.contributor.author | Rosenwald, A. | |
dc.contributor.author | Rieckmann, P. | |
dc.contributor.author | Müller-Hermelink, H.-K. | |
dc.contributor.author | Eck, M. | |
dc.date.accessioned | 2018-09-20T16:24:07Z | |
dc.date.available | 2018-09-20T16:24:07Z | |
dc.date.issued | 2008-03-05 | |
dc.identifier.citation | Schmaußer, B. Endrich, S.; Beier, D.; Moran, A. P.; Burek, C. J.; Rosenwald, A.; Rieckmann, P.; Müller-Hermelink, H.-K.; Eck, M. (2008). Triggering receptor expressed on myeloid cells-1 (trem-1) expression on gastric epithelium: implication for a role of trem-1 in helicobacter pylori infection. Clinical & Experimental Immunology 152 (1), 88-94 | |
dc.identifier.issn | 0009-9104 | |
dc.identifier.uri | http://hdl.handle.net/10379/13828 | |
dc.description.abstract | In Helicobacter pylori gastritis gastric epithelium plays a central role in the innate immunity to H. pylori. However, epithelial receptors interacting with H. pylori have been poorly characterized so far. Recently a new triggering receptor expressed on myeloid cells-1 (TREM-1) has been identified on human neutrophils and monocytes. On these cells TREM-1 triggers innate immunity by stimulating the secretion of interleukin (IL)-8 and tumour necrosis factor (TNF)-alpha and thus amplifies bacterial-induced inflammation. In this study expression and function of TREM-1 in gastric epithelium exposed to H. pylori has been investigated. TREM-1 mRNA and protein were expressed on gastric epithelial cell lines as demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence activated cell sorter analysis. Gastric epithelial TREM-1 expression was up-regulated directly by H. pylori and was independent of epithelial IL-8 induced by H. pylori. Immunohistochemistry and tissue RT-PCR demonstrated significantly stronger TREM-1 expression in H. pylori gastritis compared with the non-inflamed gastric mucosa supporting in vivo that epithelial TREM-1 is up-regulated during H. pylori infection. Stimulation of gastric epithelial TREM-1 receptor resulted in IL-8 up-regulation on mRNA and protein level, as shown by real-time PCR and immunoassay. This is the first study localizing TREM-1 on gastric epithelium. Functional data suggest that TREM-1 expressed on gastric epithelium amplifies inflammation of the underlying gastric mucosa by up-regulation of IL-8. | |
dc.publisher | Wiley-Blackwell | |
dc.relation.ispartof | Clinical & Experimental Immunology | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | gastric epithelium | |
dc.subject | h. pylori gastritis | |
dc.subject | h. pylori | |
dc.subject | innate immunity | |
dc.subject | trem-1 | |
dc.subject | inflammatory responses | |
dc.subject | cutting edge | |
dc.subject | neutrophils | |
dc.title | Triggering receptor expressed on myeloid cells-1 (trem-1) expression on gastric epithelium: implication for a role of trem-1 in helicobacter pylori infection | |
dc.type | Article | |
dc.identifier.doi | 10.1111/j.1365-2249.2008.03608.x | |
dc.local.publishedsource | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2008.03608.x/pdf | |
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