Triggering receptor expressed on myeloid cells-1 (trem-1) expression on gastric epithelium: implication for a role of trem-1 in helicobacter pylori infection
Moran, A. P.
Burek, C. J.
MetadataShow full item record
This item's downloads: 0 (view details)
Schmaußer, B. Endrich, S.; Beier, D.; Moran, A. P.; Burek, C. J.; Rosenwald, A.; Rieckmann, P.; Müller-Hermelink, H.-K.; Eck, M. (2008). Triggering receptor expressed on myeloid cells-1 (trem-1) expression on gastric epithelium: implication for a role of trem-1 in helicobacter pylori infection. Clinical & Experimental Immunology 152 (1), 88-94
In Helicobacter pylori gastritis gastric epithelium plays a central role in the innate immunity to H. pylori. However, epithelial receptors interacting with H. pylori have been poorly characterized so far. Recently a new triggering receptor expressed on myeloid cells-1 (TREM-1) has been identified on human neutrophils and monocytes. On these cells TREM-1 triggers innate immunity by stimulating the secretion of interleukin (IL)-8 and tumour necrosis factor (TNF)-alpha and thus amplifies bacterial-induced inflammation. In this study expression and function of TREM-1 in gastric epithelium exposed to H. pylori has been investigated. TREM-1 mRNA and protein were expressed on gastric epithelial cell lines as demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence activated cell sorter analysis. Gastric epithelial TREM-1 expression was up-regulated directly by H. pylori and was independent of epithelial IL-8 induced by H. pylori. Immunohistochemistry and tissue RT-PCR demonstrated significantly stronger TREM-1 expression in H. pylori gastritis compared with the non-inflamed gastric mucosa supporting in vivo that epithelial TREM-1 is up-regulated during H. pylori infection. Stimulation of gastric epithelial TREM-1 receptor resulted in IL-8 up-regulation on mRNA and protein level, as shown by real-time PCR and immunoassay. This is the first study localizing TREM-1 on gastric epithelium. Functional data suggest that TREM-1 expressed on gastric epithelium amplifies inflammation of the underlying gastric mucosa by up-regulation of IL-8.