Browsing College of Science by Author "Togashi, Denisio M."
Now showing items 1-7 of 7
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Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy
Broderick, Ronan; Ramadurai, Sivaramakrishnan; Tóth, Katalin; Togashi, Denisio M.; Ryder, Alan G.; Langowski, Jörg; Nasheuer, Heinz-Peter (Public Library of Science, 2012-04-19)Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous ... -
Cell cycle-dependent mobility of Cdc45 in living cells determined by fluorescence correlation spectroscopy.
Broderick, Ronan; Ramadurai, Sivaramakrishnan; Togashi, Denisio M.; Ryder, Alan G.; Langowski, Jorg (Public Library of Science, 2012)Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous ... -
Fluorescence study of bovine serum albumin and Ti and Sn Oxide nanoparticles interactions.
Togashi, Denisio M.; Ryder, Alan G.; Mc Mahon, Deirdre; Dunne, Peter; McManus, James (The Optical Society, 2007)Nanochemistry offers stimulating opportunities for a wide variety of applications in the biosciences. Understanding of the interaction of nanoparticles with biomolecules such as proteins is very important as it can help ... -
Investigating tryptophan quenching of fluorescein fluorescence under protolytic equilibrium
Ryder, Alan G.; Szczupak, Boguslaw; Togashi, Denisio M.; Calvet, Amandine; O'Loughlin, Muireann (2009)Fluorescein is one of most used fluorescent labels for characterizing biological systems, such as proteins, and is used in fluorescence microscopy. However, if fluorescein is to be used for quantitative measurements involving ... -
Measuring the micro-polarity and hydrogen-bond donor/acceptor ability of thermoresponsive N-isopropylacrylamide/N-tert-butylacrylamide copolymer films using solvatochromic indicators
Szczupak, Boguslaw; Ryder, Alan G.; Togashi, Denisio M.; Rochev, Yuri A.; Glynn, Thomas J. (application/pdf, 2009)Thin polymer films are important in many areas of biomaterials research, biomedical devices, and biological sensors. The accurate in situ measurement of multiple physicochemical properties of thin polymer films is critical ... -
Measuring the Micro-Polarity and Hydrogen-Bond Donor/Acceptor Ability of Thermoresponsive N-Isopropylacrylamide/N-tert-Butylacrylamide Copolymer Films Using Solvatochromic Indicators
Ryder, Alan G.; Szczupak, Boguslaw; Togashi, Denisio M.; Rochev, Yuri A.; Glynn, Thomas J. (2009)Thin polymer films are important in many areas of biomaterials research, biomedical devices, and biological sensors. The accurate in situ measurement of multiple physicochemical properties of thin polymer films is critical ... -
Monitoring local unfolding of bovine serum albumin during denaturation using steady-state and time-resolved fluorescence spectroscopy
Togashi, Denisio M.; Ryder, Alan G.; O'Shaughnessy, Domhnall (Springer, 2010-03)In a previous report (J. Fluoresc. 16, 153, 2006) we studied the chaotropiclly induced denaturation of Bovine Serum Albumin (BSA) using the fluorescence decay kinetics at different stages in the denaturation of BSA by ...