Browsing College of Science by Author "http://dx.doi.org/10.13039/501100008530"
Now showing items 1-20 of 32
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Analyzing protein conjugation reactions for antibody-drug conjugate synthesis using polarized excitation emission matrix spectroscopy
de Faria e Silva, Ana L.; Ryder, Alan G. (Wiley, 2022-09-07)Antibody-drug conjugates (ADCs) are promising anticancer therapeutics, which offer important advantages compared to more classical therapies. There are a variety of ADC critical quality attributes (CQAs) such as the protein ... -
Anomer preferences for glucuronic and galacturonic acid and derivatives and influence of electron-withdrawing substituents
Kerins, Louise; Byrne, Sylvester; Gabba, Adele; Murphy, Paul V. (American Chemical Society, 2018-07-04)Equilibrium anomeric ratios are reported for pyranoses (hemiacetals) of glucuronic and galacturonic acid and their derivatives. These are compared to related gluco- and galactopyranoses and to deoxyfluorogluco- and ... -
Building blocks from monosaccharides for synthesis of scaffolds, including macrocycles. Application of allylic azide rearrangement, azide-alkyne cycloaddition and ring closing metathesis
Fox, Karen A.; Chadda, Rekha; Cardona, Francisco; Barron, Stephen; McArdle, Patrick; Murphy, Paul V. (Elsevier, 2020-09-18)Synthesis of compounds with characteristics of natural products are required to increase the diversity and biological relevance of compounds for screening. These include new frameworks/scaffolds, with multiple stereogenic ... -
Calixarene-mediated assembly of a small antifungal protein
Alex, Jimi M.; Rennie, Martin L.; Engilberge, Sylvain; Lehoczki, Gábor; Dorottya, Hajdu; Fizil, Ádám; Batta, Gyula; Crowley, Peter B. (International Union of Crystallography, 2019-02-05)Synthetic macrocycles such as calixarenes and cucurbiturils are increasingly applied as mediators of protein assembly and crystallization. The macrocycle can facilitate assembly by providing a surface on which two or more ... -
Cephalopod recipes 2021: 21 recipes for octopus, cuttlefish and squid from Spain, France, Ireland and Portugal
Roumbedakis, C.; Amiero, J.; Pita, C.; Longo, C.; Montero, C.; Mendes, R.; Matos, F.; Monteiro, S.; Larivain, A.; Davoren, Sadie; O'Leary, A.; Lago, R.; Romón, J.; Pierce, G. J.; Power, Anne Marie (Cephs and Chefs (Interreg Atlantic Area Community Engagement Publications), 2021)[No abstract available] -
Characterization of Lysozyme PEGylation products using polarized Excitation Emission Matrix (pEEM) spectroscopy
de Faria E Silva, Ana Luiza; Elcoroaristizabal, Saioa; Ryder, Alan G. (Wiley, 2020-07-07)The growing use of therapeutic proteins requires accurate measurement techniques for measuring biophysical and structural changes during manufacturing. This is particularly true for PEGylation of proteins, because ... -
Conversion of gel-forming crystal needles to easily processable more equant crystals Using high-shear ultralow attrition agitation: accelerated Ostwald ripening without crystal attrition
Civati, Francesco; Erxleben, Andrea; Kellehan, Seamus; McArdle, Patrick (American Chemical Society, 2019-02-01)We demonstrate the first examples of the transformation of gel-forming crystal needles into easily processable more equant crystals by accelerated Ostwald ripening without detectable crystal attrition. Using high solution ... -
Crystallization of organic salts from the gas phase: When does proton transfer take place?
O’Malley, Ciarán; Erxleben, Andrea; McArdle, Patrick; Simmie, John M. (American Chemical Society, 2020-12-13)Salt formation with proton transfer is observed in crystals grown by cosublimation of the salt coformers. Diflunisal salts were obtained with 4-[3-(pyridin-4-yl)propyl]pyridine, piperazine, and 4-dimethylaminopyridine. ... -
Design of experiments approach to provide enhanced glucose-oxidising enzyme electrode for membrane-less enzymatic fuel cells operating in human physiological fluids
Bennett, Richard; Osadebe, Isioma; Kumar, Rakesh; Ó Conghaile, Peter; Leech, Dónal (Wiley, 2018-03-30)Graphite electrodes are modified with a redox polymer, [Os(4,4-dimethoxy-2,2-bipyridine)(2)(polyvinylimidazole)(10)Cl](+) (E degrees=-0.02V vs Ag/AgCl (3M KCl), crosslinked with a flavin adenine dinucleotide glucose ... -
Development of a rapid Polarized Total Synchronous Fluorescence Spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth
Boateng, Bernard O.; Elcoroaristizabal, Saioa; Ryder, Alan G. (Wiley, 2021-01-27)Protein quantification during bioprocess monitoring is essential for biopharmaceutical manufacturing and is complicated by the complex chemical composition of the bioreactor broth. Here we present the early-stage development ... -
Evaluating the interaction of human serum albumin (HSA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes in different aqueous environments using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
Gordon, Fiona; Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2022-01-07)Studying the interaction between plasma proteins and liposomes is critical, particularly for their use as drug delivery systems. Here, the efficacy of anisotropy resolved multidimensional emission spectroscopy (ARMES) for ... -
Experimental assessment of clinical MRI-induced global SAR distributions in head phantoms
Blackwell, James; Oluniran, Gideon; Tuohy, Brendan; Destrade, Michel; Kraśny, Marcin Jan; Colgan, Niall (Elsevier, 2019-10-05)Accurate estimation of SAR is critical to safeguarding vulnerable patients who require an MRI procedure. The increased static field strength and RF duty cycle capabilities in modern MRI scanners mean that systems can easily ... -
Influence of acyl groups on glucopyranoside reactivity in Lewis acid promoted anomerisation
Farrell, Mark P.; Doyle, Lisa M.; Murphy, Paul V. (Elsevier, 2018-05-26)Lewis acid promoted anomerisation has potential in O- or S-glycoside synthesis. Herein, the anomerisation kinetics of thirty-one β-d-glucopyranosides was determined to determine how particular acyl protecting groups and ... -
Investigating native state fluorescence emission of Immunoglobulin G using polarized Excitation Emission Matrix (pEEM) spectroscopy and PARAFAC
Steiner-Browne, Marina; Elcoroaristizabal, Saioa; Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2018-12-20)Intrinsic fluorescence spectroscopy (IFS) measurements for protein structural analysis can be enhanced by the use of anisotropy resolved multidimensional emission spectroscopy (ARMES). ARMES attempts to overcome the ... -
Lewis acid promoted anomerisation of alkyl O- and S-xylo-, arabino- and fucopyranosides
Doyle, Lisa M.; Meany, Fiach B.; Murphy, Paul V. (Elsevier, 2018-11-24)Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides. Lewis acid promoted anomerisation of some of their ... -
Manipulating cocrystal size and morphology using a combination of temperature cycling and additives
Civati, Francesco; Svoboda, Vaclav; Urwin, Stephanie Jane; McArdle, Patrick; Erxleben, Andrea; Croker, D.; ter Horst, Joop (American Chemical Society, 2020-11-20)A cooling crystallization of benzoic acid and isonicotinamide in ethanol yields the 1:1 cocrystal with an extreme needle-like morphology with an initial mean aspect ratio of ∼10 and a size of ∼64 μm. We demonstrate that ... -
Microplastics in corals: An emergent threat
de Oliveira Soares, Marcelo; Matos, Eliana; Lucas, Caroline; Rizzo, Lucia; Allcock, A. Louise; Rossi, Sergio (Elsevier, 2020-11-01)This article seeks to present a summary of knowledge and thus improve awareness of microplastic impacts on corals. Recent research suggests that microplastics have a variety of species-specific impacts. Among them, a reduced ... -
Modelling Förster resonance energy transfer (FRET) using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
Gordon, Fiona; Elcoroaristizabal, Saioa; Ryder, Alan G. (Elsevier, 2020-11-16)Background Förster Resonance Energy Transfer (FRET) is widely used to study the structure and dynamics of biomolecular systems and also causes the non-linear fluorescence response observed in multi-fluorophore proteins. ... -
Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy
de Faria E Silva, Ana Luiza; Elcoroaristizabal, Saioa; Ryder, Alan G. (Elsevier, 2019-12-10)Immunoglobulin G (IgG) is often used as a starting material for the production of functionalised antibodies, like Antibody Drug Conjugates (ADCs), PEGlyated-conjugates, or radioimmunoconjugates. The gross structural quality ... -
North Eastern Atlantic cephalopods stock assessment in a data limited framework Surplus Production in a Continuous Time (SPiCT): Annex1to WP.4.2deliverable
Larivain, Angela; Petroni, Michael; Iriondo, Ane; Abad, Esther; Valeiras, Julio; Moreno, Ana; Dinis, David; Rocha, Alberto; Laptikovsky, Vladimir; Robin, Jean-Paul; Power, Anne Marie (Cephs and Chefs (Interreg Atlantic Area Community Engagement Publications), 2021)The lack of management leaves fishery resources vulnerable to increases in fishing pressure.Although some cephalopods are of commercial importance, most of Northeast Atlantic stocks are non-quota ...