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dc.contributor.authorRusling, James F.
dc.contributor.authorWasalathanthri, Dhanuka P.
dc.contributor.authorSchenkman, John B.
dc.date.accessioned2018-09-20T16:23:27Z
dc.date.available2018-09-20T16:23:27Z
dc.date.issued2014-01-01
dc.identifier.citationRusling, James F. Wasalathanthri, Dhanuka P.; Schenkman, John B. (2014). Thin multicomponent films for functional enzyme devices and bioreactor particles. Soft Matter 10 (41), 8145-8156
dc.identifier.issn1744-683X,1744-6848
dc.identifier.urihttp://hdl.handle.net/10379/13734
dc.description.abstractComplex functional films containing enzymes and other biomolecules are easily fabricated in nm-scale thicknesses by using layer-by-layer (LbL) methodologies first popularized by Lvov and Decher. In this review, we highlight the high level functional capabilities possible with LbL films of biomolecules based on our own research experiences. We first describe the basics of enzyme film fabrication by LbL alternate electrostatic adsorption, then discuss how to make functional enzyme-polyion films of remarkably high stability. Focusing on cytochrome P450s, we discuss films developed to electrochemically activate the natural catalytic cycle of these key metabolic enzymes. We then describe multifunctional, multicomponent DNA/enzyme/polyion films on arrays and particle surfaces for high throughput metabolic toxicity screening using electrochemiluminescence and LC-MS/MS. Using multicomponent LbL films, complex functionality for bioanalytical and biochemical purposes can be achieved that is difficult or impossible using conventional approaches.
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.ispartofSoft Matter
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectlayer-by-layer
dc.subjectquartz-crystal microbalance
dc.subjectultrathin multilayer films
dc.subjectself-assembly process
dc.subjectpolyion films
dc.subjecthuman cytochrome-p450
dc.subjectbiocolloid reactors
dc.subjecttoxicity assessment
dc.subjectcharged surfaces
dc.subjectdrug discovery
dc.titleThin multicomponent films for functional enzyme devices and bioreactor particles
dc.typeArticle
dc.identifier.doi10.1039/c4sm01679c
dc.local.publishedsourcehttp://europepmc.org/articles/pmc4183705?pdf=render
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland