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    Thin multicomponent films for functional enzyme devices and bioreactor particles

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    Date
    2014-01-01
    Author
    Rusling, James F.
    Wasalathanthri, Dhanuka P.
    Schenkman, John B.
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    Cited 21 times in Scopus (view citations)
    
    Recommended Citation
    Rusling, James F. Wasalathanthri, Dhanuka P.; Schenkman, John B. (2014). Thin multicomponent films for functional enzyme devices and bioreactor particles. Soft Matter 10 (41), 8145-8156
    Published Version
    http://europepmc.org/articles/pmc4183705?pdf=render
    Abstract
    Complex functional films containing enzymes and other biomolecules are easily fabricated in nm-scale thicknesses by using layer-by-layer (LbL) methodologies first popularized by Lvov and Decher. In this review, we highlight the high level functional capabilities possible with LbL films of biomolecules based on our own research experiences. We first describe the basics of enzyme film fabrication by LbL alternate electrostatic adsorption, then discuss how to make functional enzyme-polyion films of remarkably high stability. Focusing on cytochrome P450s, we discuss films developed to electrochemically activate the natural catalytic cycle of these key metabolic enzymes. We then describe multifunctional, multicomponent DNA/enzyme/polyion films on arrays and particle surfaces for high throughput metabolic toxicity screening using electrochemiluminescence and LC-MS/MS. Using multicomponent LbL films, complex functionality for bioanalytical and biochemical purposes can be achieved that is difficult or impossible using conventional approaches.
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    http://hdl.handle.net/10379/13734
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