dc.contributor.author | Rosberg-Cody, E. | |
dc.contributor.author | Stanton, C. | |
dc.contributor.author | O'Mahony, L. | |
dc.contributor.author | Wall, R. | |
dc.contributor.author | Shanahan, F. | |
dc.contributor.author | Quigley, E. M. | |
dc.contributor.author | Fitzgerald, G. F. | |
dc.contributor.author | Ross, R. P. | |
dc.date.accessioned | 2018-09-20T16:23:13Z | |
dc.date.available | 2018-09-20T16:23:13Z | |
dc.date.issued | 2010-12-22 | |
dc.identifier.citation | Rosberg-Cody, E. Stanton, C.; O'Mahony, L.; Wall, R.; Shanahan, F.; Quigley, E. M.; Fitzgerald, G. F.; Ross, R. P. (2010). Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice. Microbiology 157 , 609-615 | |
dc.identifier.issn | 1350-0872,1465-2080 | |
dc.identifier.uri | http://hdl.handle.net/10379/13703 | |
dc.description.abstract | We have previously demonstrated that oral administration of a metabolically active Bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (CLA), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 CLA and omega-3 (n-3) fatty acids in liver and adipose tissue. In this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production of t10, c12 CLA) from Propionibacterium acnes (PAI) could influence the fatty acid composition of different tissues in a mouse model. Linoleic-acid-supplemented diets (2 %, w/w) were fed in combination with either a recombinant t10, c12 CLA-producing Lactobacillus paracasei NFBC 338 (Lb338), or an isogenic (vector-containing) control strain, to BALB/c mice for 8 weeks. A third group of mice received linoleic acid alone (2 %, w/w). Tissue fatty acid composition was assessed by GLC at the end of the trial. Ingestion of the strain expressing linoleic acid isomerase was associated with a 4-fold increase (P < 0.001) in t10, c12 CLA in adipose tissues of the mice when compared with mice that received the isogenic non-CLA-producing strain. The livers of the mice that received the recombinant CLA-producing Lb338 also contained a 2.5-fold (albeit not significantly) higher concentration of t10, c12 CLA, compared to the control group. These data demonstrate that a single gene (encoding linoleic acid isomerase) expressed in an intestinal microbe can influence the fatty acid composition of host fat. | |
dc.publisher | Microbiology Society | |
dc.relation.ispartof | Microbiology | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | colon-cancer cells | |
dc.subject | induced obese mice | |
dc.subject | body-fat | |
dc.subject | serum-lipids | |
dc.subject | microbiota | |
dc.subject | supplementation | |
dc.subject | bacteria | |
dc.subject | trans-10 | |
dc.subject | liver | |
dc.subject | diet | |
dc.title | Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice | |
dc.type | Article | |
dc.identifier.doi | 10.1099/mic.0.043406-0 | |
dc.local.publishedsource | http://mic.microbiologyresearch.org/deliver/fulltext/micro/157/2/609.pdf?itemId=/content/journal/micro/10.1099/mic.0.043406-0&mimeType=pdf&isFastTrackArticle= | |
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