Identification and functional analysis of secreted effectors from phytoparasitic nematodes
Gupta, Vijai K.
Goyal, Aakash K.
MetadataShow full item record
This item's downloads: 0 (view details)
Cited 30 times in Scopus (view citations)
Rehman, Sajid; Gupta, Vijai K. Goyal, Aakash K. (2016). Identification and functional analysis of secreted effectors from phytoparasitic nematodes. BMC Microbiology 16 ,
Background: Plant parasitic nematodes develop an intimate and long-term feeding relationship with their host plants. They induce a multi-nucleate feeding site close to the vascular bundle in the roots of their host plant and remain sessile for the rest of their life. Nematode secretions, produced in the oesophageal glands and secreted through a hollow stylet into the host plant cytoplasm, are believed to play key role in pathogenesis. To combat these persistent pathogens, the identity and functional analysis of secreted effectors can serve as a key to devise durable control measures. In this review, we will recapitulate the knowledge over the identification and functional characterization of secreted nematode effector repertoire from phytoparasitic nematodes. Research: Despite considerable efforts, the identity of genes encoding nematode secreted proteins has long been severely hampered because of their microscopic size, long generation time and obligate biotrophic nature. The methodologies such as bioinformatics, protein structure modeling, in situ hybridization microscopy, and protein-protein interaction have been used to identify and to attribute functions to the effectors. In addition, RNA interference (RNAi) has been instrumental to decipher the role of the genes encoding secreted effectors necessary for parasitism and genes attributed to normal development. Recent comparative and functional genomic approaches have accelerated the identification of effectors from phytoparasitic nematodes and offers opportunities to control these pathogens. Conclusion: Plant parasitic nematodes pose a serious threat to global food security of various economically important crops. There is a wealth of genomic and transcriptomic information available on plant parasitic nematodes and comparative genomics has identified many effectors. Bioengineering crops with dsRNA of phytonematode genes can disrupt the life cycle of parasitic nematodes and therefore holds great promise to develop resistant crops against plant-parasitic nematodes.
Showing items related by title, author, creator and subject.
Nematode associates and susceptibility of a protected slug (geomalacus maculosus) to four biocontrol nematodes Carnaghi, Manuela; Rae, Robbie; De Ley, Irma Tandingan; Johnston, Erin; Kindermann, Gesche; Mc Donnell, Rory; O’Hanlon, Aidan; Reich, Inga; Sheahan, Jerome; Williams, Christopher D.; Gormally, Michael J. (Informa UK Limited, 2017-02-01)The impact of selected entomopathogenic nematodes and Phasmarhabditis hermaphrodita on the European-Union-protected slug Geomalacus maculosus and the sympatric Lehmannia marginata was investigated. There was no significant ...
Nematode response to nitrogen and phosphorus in grasslands, assessed by microscopy and molecular methods Chen, Xiaoyun (2012-09-19)Although nematode community analysis is considered to be a useful environmental indicator, a major impediment to its wider adoption is the need for detailed morphological identification. Molecular techniques offer an ...
Response to teladorsagia circumcincta infection in scottish blackface lambs with divergent phenotypes for nematode resistance McRae, Kathryn M.; Good, Barbara; Hanrahan, James P.; Glynn, Assumpta; O’Connell, Mary J.; Keane, Orla M. (Elsevier BV, 2014-12-01)