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dc.contributor.authorRedondo, Juan Alfonso
dc.contributor.authorMartínez-Campos, Enrique
dc.contributor.authorPlet, Laetitia
dc.contributor.authorPérez-Perrino, Mónica
dc.contributor.authorNavarro, Rodrigo
dc.contributor.authorCorrales, Guillermo
dc.contributor.authorPandit, Abhay
dc.contributor.authorReinecke, Helmut
dc.contributor.authorGallardo, Alberto
dc.contributor.authorLópez-Lacomba, José Luis
dc.contributor.authorFernández-Mayoralas, Alfonso
dc.contributor.authorElvira, Carlos
dc.date.accessioned2018-09-20T16:22:36Z
dc.date.available2018-09-20T16:22:36Z
dc.date.issued2016-02-02
dc.identifier.citationRedondo, Juan Alfonso; Martínez-Campos, Enrique; Plet, Laetitia; Pérez-Perrino, Mónica; Navarro, Rodrigo; Corrales, Guillermo; Pandit, Abhay; Reinecke, Helmut; Gallardo, Alberto; López-Lacomba, José Luis; Fernández-Mayoralas, Alfonso; Elvira, Carlos (2016). Polymeric gene carriers bearing pendant β-cyclodextrin: the relevance of glycoside permethylation on the “in vitro” cell response. Macromolecular Rapid Communications 37 (7), 575-583
dc.identifier.issn1022-1336
dc.identifier.urihttp://hdl.handle.net/10379/13616
dc.description.abstractThe incorporation of cyclodextrins (CDs) to nonviral cationic polymer vectors is very attractive due to recent studies that report a clear improvement of their cytocompatibility and transfection efficiency. However, a systematic study on the influence of the CD derivatization is still lacking. In this work, the relevance of beta-CD permethylation has been addressed by preparing and evaluating two series of copolymers of the cationic N-ethyl pyrrolidine methacrylamide (EPA) and styrenic units bearing pendant hydroxylated and permethylated beta-CDs (HCDSt and MeCDSt, respectively). For both cell lines, CDs permethylation shows a strong influence on plasmid DNA complexation, "in vitro" cytocompatibility and transfection efficiency of the resulting copolymers over two murine cell lines. While the incorporation of the hydroxylated CD moiety increased the cytotoxicity of the copolymers in comparison with their homopolycationic counterpart, the permethylated copolymers have shown full cytocompatibility as well as superior transfection efficiency than the controls. This behavior has been related to the different chemical nature of both units and tentatively to a different distribution of units along the polymeric chains. Cellular internalization analysis with fluorescent copolymers supports this behavior.
dc.publisherWiley-Blackwell
dc.relation.ispartofMacromolecular Rapid Communications
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectcationic polymers
dc.subjectcyclodextrins
dc.subjectgene therapy
dc.subjectnonviral gene vectors
dc.subjectpolyamidoamine dendrimer
dc.subjectalpha-cyclodextrin
dc.subjectdelivery vectors
dc.subjectgamma-cyclodextrins
dc.subjectcationic polymers
dc.subjectclick clusters
dc.subjecttransfection
dc.subjectpyrrolidine
dc.subjecttherapy
dc.subjectcholesterol
dc.titlePolymeric gene carriers bearing pendant β-cyclodextrin: the relevance of glycoside permethylation on the “in vitro” cell response
dc.typeArticle
dc.identifier.doi10.1002/marc.201500647
dc.local.publishedsourcehttps://digital.csic.es/bitstream/10261/136191/1/accesoRestringido.pdf
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