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dc.contributor.authorQuinn, Brian
dc.contributor.authorCostello, Mark J.
dc.contributor.authorDorange, Germaine
dc.contributor.authorWilson, James G.
dc.contributor.authorMothersill, Carmel
dc.date.accessioned2018-09-20T16:22:08Z
dc.date.available2018-09-20T16:22:08Z
dc.date.issued2009-03-01
dc.identifier.citationQuinn, Brian; Costello, Mark J. Dorange, Germaine; Wilson, James G.; Mothersill, Carmel (2009). Development of an in vitro culture method for cells and tissues from the zebra mussel (dreissena polymorpha). Cytotechnology 59 (2), 121-134
dc.identifier.issn0920-9069,1573-0778
dc.identifier.urihttp://hdl.handle.net/10379/13550
dc.description.abstractDespite the successful transfer of mammalian in vitro techniques for use with fish and other vertebrates, little progress has been made in the area of invertebrate tissue culture. This paper describes the development of an in vitro technique for the culture of both cells in suspension and tissue explants from the gill, digestive gland and mantle of the zebra mussel (Dreissena polymorpha) and their successful maintenance in culture for up to 14 days. Cell suspensions from the gills and digestive gland were the most successful technique developed with viability > 80% maintained for up to 8 days in culture, suitable for use in short term toxicity tests. Tissue explants from the mantle were also maintained in culture for up to 14 days. This paper describes the challenges involved in the development of a novel in vitro culture technique for aquatic invertebrates.
dc.publisherSpringer Nature
dc.relation.ispartofCytotechnology
dc.subjectin vitro
dc.subjectcell culture
dc.subjectinvertebrate
dc.subjectzebra mussel
dc.subjectpecten-maximus l.
dc.subjectdigestive gland
dc.subjectmytilus-galloprovincialis
dc.subjectcrassostrea-virginica
dc.subjecttoxicity
dc.subjectpollution
dc.subjectcadmium
dc.subjectoyster
dc.subjectgrowth
dc.subjectmedia
dc.titleDevelopment of an in vitro culture method for cells and tissues from the zebra mussel (dreissena polymorpha)
dc.typeArticle
dc.identifier.doi10.1007/s10616-009-9202-3
dc.local.publishedsourcehttp://europepmc.org/articles/pmc2698444?pdf=render
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