Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production
Passari, Ajit Kumar
Mishra, Vineet Kumar
Gupta, Vijai Kumar
Sharma, Rupak Kumar
Donovan, Anthonia O’.
Singh, Bhim Pratap
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Passari, Ajit Kumar; Mishra, Vineet Kumar; Singh, Garima; Singh, Pratibha; Kumar, Brijesh; Gupta, Vijai Kumar; Sharma, Rupak Kumar; Saikia, Ratul; Donovan, Anthonia O’. Singh, Bhim Pratap (2017). Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production. Scientific Reports 7 ,
Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. In the present investigation, 169 endophytic actinobacteria were isolated from endospheric tissues of Rhynchotoechum ellipticum. Based on their antimicrobial potential, 81 strains were identified by 16rRNA gene analysis, which were taxonomically grouped into 15 genera. All identified strains were screened for their plant growth promoting attributes and, for the presence of modular polyketide synthases (PKSI, PKSII and nonribosomal peptide synthetase (NRPS) gene clusters to correlate the biosynthetic genes with their functional properties. Expression studies and antioxidant potential for four representative strains were evaluated using qRT-PCR and DPPH assay respectively. Additionally, six antibiotics (erythromycin, ketoconazole, fluconazole, chloramphenicol, rifampicin and miconazole) and nine phenolic compounds (catechin, kaempferol, chebulagic acid, chlorogenic acid, Asiatic acid, ferulic acid, arjunic acid, gallic acid and boswellic acid) were detected and quantified using UHPLC-QqQLIT-MS/MS. Furthermore, three strains (BPSAC77, 121 and 101) showed the presence of the anticancerous compound paclitaxel which was reported for the first time from endophytic actinobacteria. This study provides a holistic picture, that endophytic actinobacteria are rich bacterial resource for bioactive natural products, which has a great prospective in agriculture and pharmaceutical industries.
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Author correction: insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production Passari, Ajit Kumar; Mishra, Vineet Kumar; Singh, Garima; Singh, Pratibha; Kumar, Brijesh; Gupta, Vijai Kumar; Sarma, Rupak Kumar; Saikia, Ratul; Donovan, Anthonia O’.; Singh, Bhim Pratap (Springer Nature, 2018-03-12)
Determination and production of antimicrobial compounds by aspergillus clavatonanicus strain mj31, an endophytic fungus from mirabilis jalapa l. using uplc-esi-ms/ms and td-gc-ms analysis Mishra, Vineet Kumar; Passari, Ajit Kumar; Chandra, Preeti; Leo, Vincent Vineeth; Kumar, Brijesh; Uthandi, Sivakumar; Thankappan, Sugitha; Gupta, Vijai Kumar; Singh, Bhim Pratap (Public Library of Science (PLoS), 2017-10-19)Endophytic fungi associated with medicinal plants are reported as potent producers of diverse classes of secondary metabolites. In the present study, an endophytic fungi, Aspergillus clavatonanicus strain MJ31, exhibiting ...
In vitro and in vivo plant growth promoting activities and dna fingerprinting of antagonistic endophytic actinomycetes associates with medicinal plants Passari, Ajit Kumar; Mishra, Vineet Kumar; Gupta, Vijai Kumar; Yadav, Mukesh Kumar; Saikia, Ratul; Singh, Bhim Pratap (Public Library of Science (PLoS), 2015-09-30)Endophytic actinomycetes have shown unique plant growth promoting as well as antagonistic activity against fungal phytopathogens. In the present study forty-two endophytic actinomycetes recovered from medicinal plants were ...