Proinflammatory action of the antiinflammatory drug infliximab in tumor necrosis factor receptor-associated periodic syndrome
Hitman, Graham A.
Coughlan, Robert J.
McDermott, Michael F.
Turner, Mark D.
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Nedjai, Belinda; Hitman, Graham A. Quillinan, Niamh; Coughlan, Robert J.; Church, Leigh; McDermott, Michael F.; Turner, Mark D. (2009). Proinflammatory action of the antiinflammatory drug infliximab in tumor necrosis factor receptor-associated periodic syndrome. Arthritis & Rheumatism 60 (2), 619-625
Objective. Tumor necrosis factor receptor (TNFR) -associated periodic syndrome (TRAPS) is an autosomal-dominant autoinflammatory condition caused by mutations in the TNFRSFIA gene. Unlike other autoinflammatory diseases in which anti-TNF therapy is largely a successful treatment option, therapy with the anti-TNF drug infliximab is often ineffective in patients with TRAPS. Moreover, in certain cases, infliximab actually triggers severe episodes of inflammation. The aim of this study was to elucidate the mechanisms underlying such a reaction. Methods. Peripheral blood mononuclear cells (PBMCs) were obtained from patients with TRAPS. Both caspase 3 activity and NF-kappa B subunit activity were determined by enzyme-linked immunosorbent assay. Cytokine secretion was assessed using a specific customized human multiplex bead immunoassay kit. Results. Unlike findings in controls, cells from a family of 9 patients, all of whom carried the T50M mutation in TNFRSFIA, failed to respond to infliximab through proapoptotic induction of caspase 3 activity. Instead, we observed enhanced antiapoptotic c-Rel subunit activity, accompanied by a significant increase in secretion of the proinflammatory cytokines interleukin-1 beta (IL-1 beta), IL-1 receptor, IL-6, IL-8, and IL-12. Conclusion. Altered extracellular conformation of TNFRI, resulting from the T50M mutation in TNFRSFIA, results in failure of PBMCs to induce an apoptotic response to infliximab. We hypothesize that failure to shed infliximab-bound TNF/TNFRI from the cell surface of cells from patients with the T50M mutation triggers c-Rel activation, and that this leads to a marked increase in cytokine secretion and an increased proinflammatory response. In light of these findings, we strongly advise caution when prescribing infliximab as anti-TNF therapy to patients with TRAPS.