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dc.contributor.authorMnich, Eliza
dc.contributor.authorKowalewicz-Kulbat, Magdalena
dc.contributor.authorSicińska, Paulina
dc.contributor.authorHinc, Krzysztof
dc.contributor.authorObuchowski, Michał
dc.contributor.authorGajewski, Adrian
dc.contributor.authorMoran, Anthony P
dc.contributor.authorChmiela, Magdalena
dc.identifier.citationMnich, Eliza; Kowalewicz-Kulbat, Magdalena; Sicińska, Paulina; Hinc, Krzysztof; Obuchowski, Michał; Gajewski, Adrian; Moran, Anthony P; Chmiela, Magdalena (2016). Impact ofhelicobacter pylorion the healing process of the gastric barrier. World Journal of Gastroenterology 22 (33), 7536-7558
dc.description.abstractAIM To determine the impact of selected well defined Helicobacter pylori (H. pylori) antigens on gastric barrier cell turnover. METHODS In this study, using two cellular models of gastric epithelial cells and fibroblasts, we have focused on exploring the effects of well defined H. pylori soluble components such as glycine acid extract antigenic complex (GE), subunit A of urease (UreA), cytotoxin associated gene A protein (CagA) and lipopolysaccharide (LPS) on cell turnover by comparing the wound healing capacity of the cells in terms of their proliferative and metabolic activity as well as cell cycle distribution. Toxic effects of H. pylori components have been assessed in an association with damage to cell nuclei and inhibition of signal transducer and activator of transcription 3 (STAT3) phosphorylation. RESULTS We showed that H. pylori GE, CagA and UreA promoted regeneration of epithelial cells and fibroblasts, which is necessary for effective tissue healing. However, in vivo increased proliferative activity of these cells may constitute an increased risk of gastric neoplasia. In contrast, H. pylori LPS showed a dose-dependent influence on the process of wound healing. At a low concentration (1 ng/mL) H. pylori LPS accelerated of healing epithelial cells, which was linked to significantly enhanced cell proliferation and MTT reduction as well as lack of alterations in cell cycle and downregulation of epidermal growth factor (EGF) production as well as cell nuclei destruction. By comparison, H. pylori LPS at a high concentration (25 ng/mL) inhibited the process of wound repair, which was related to diminished proliferative activity of the cells, cell cycle arrest, destruction of cell nuclei and downregulation of the EGF/STAT3 signalling pathway. CONCLUSION In vivo H. pylori LPS driven effects might lead to the maintenance of chronic inflammatory response and pathological disorders on the level of the gastric mucosal barrier.
dc.publisherBaishideng Publishing Group Inc.
dc.relation.ispartofWorld Journal of Gastroenterology
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjecthelicopter pylori
dc.subjectwound healing
dc.subjectgastric barrier dysfunction
dc.subjectepithelial-cell proliferation
dc.subjectouter-membrane vesicles
dc.subjectalpha-induced apoptosis
dc.subjectblood-group antigens
dc.subjectpotential role
dc.subjectvacuolating cytotoxin
dc.titleImpact ofhelicobacter pylorion the healing process of the gastric barrier

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