dc.contributor.author | Mnich, Katarzyna | en |
dc.contributor.author | Finn, David P. | en |
dc.contributor.author | Dowd, Eilis | en |
dc.contributor.author | Gorman, Adrienne M. | en |
dc.date.accessioned | 2010-08-20T10:32:04Z | en |
dc.date.available | 2010-08-20T10:32:04Z | en |
dc.date.issued | 2010 | en |
dc.identifier.citation | Mnich, K., Finn, D., Dowd, E., & Gorman, A. (2010) Inhibition by anandamide of 6-hydroxydopamine-induced cell death in PC12 cells. Int J Cell Biol, 2010, 818497. | en |
dc.identifier.uri | http://hdl.handle.net/10379/1276 | en |
dc.description.abstract | 6-hydroxydopamine (6-OHDA) is a selective neurotoxin that is widely used to investigate cell death and protective strategies in models of Parkinson's disease. Here, we investigated the effects of the endogenous cannabinoid, anandamide, on 6-OHDA-induced toxicity in rat adrenal phaeochromocytoma PC12 cells. Morphological analysis and caspase-3 activity assay revealed that anandamide inhibited 6-OHDA-induced apoptosis. The protection was not affected by antagonists of either cannabinoid receptors (CB1 or CB2) or the vanilloid receptor TRPV1. Anandamide-dependent protection was reduced by pretreatment with LY294002 (inhibitor of phosphatidylinositol 3-kinase, PI3K) and unaffected by U0126 (inhibitor of extracellularly-regulated kinase). Interestingly, phosphorylation of c-Jun-NH2-terminal kinase (JNK) in cells exposed to 6-OHDA was strongly reduced by anandamide pre-treatment. Furthermore, 6-OHDA induced c-Jun activation and increased Bim expression, both of which were inhibited by anandamide. Together, these data demonstrate antiapoptotic effects of anandamide and also suggest a role for activation of PI3K and inhibition of JNK signalling in anandamide-mediated protection against 6-OHDA. | en |
dc.format | application/pdf | en |
dc.language.iso | en | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Pharmacology | en |
dc.title | Inhibition by Anandamide of 6-Hydroxydopamine-Induced Cell Death in PC12 Cells | en |
dc.type | Article | en |
dc.local.publishedsource | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2825649 | en |
dc.description.peer-reviewed | peer-reviewed | en |
dc.contributor.funder | Irish Research Council for Science Engineering and Technology | en |
dc.contributor.funder | Science Foundation Ireland | en |
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