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dc.contributor.authorMcCoy, Gary R.
dc.contributor.authorKegel, Jessica U.
dc.contributor.authorTouzet, Nicolas
dc.contributor.authorFleming, Gerard T.A.
dc.contributor.authorMedlin, Linda K.
dc.contributor.authorRaine, Robin
dc.date.accessioned2018-09-20T16:16:44Z
dc.date.available2018-09-20T16:16:44Z
dc.date.issued2014-02-03
dc.identifier.citationMcCoy, Gary R. Kegel, Jessica U.; Touzet, Nicolas; Fleming, Gerard T.A.; Medlin, Linda K.; Raine, Robin (2014). An assessment of rna content inprymnesium parvum,prymnesium polylepis,cf.chattonellasp. andkarlodinium veneficumunder varying environmental conditions for calibrating an rna microarray for species detection. FEMS Microbiology Ecology 88 (1), 140-159
dc.identifier.issn0168-6496
dc.identifier.urihttp://hdl.handle.net/10379/12738
dc.description.abstractTraditional methods of identification and enumeration can be somewhat ambiguous when identifying phytoplankton that requires electron microscopic examination to verify specific morphological features. Members of the genus Prymnesium (division Haptophyta), members of the Raphidophyceae and naked dinoflagellates are examples of such phytoplankton whose identification can be difficult. One alternative to traditional microscopy-based methods of identification is to use molecular protocols to detect target species. Methods that measure cellular DNA and RNA content can be used to estimate the number of cells present in a sample. This study investigated the variation of RNA yields in Prymnesium parvum, P.polylepis, cf. Chattonella sp. and Karlodinium veneficum cells grown under different light, temperature, salinity and inorganic nutrient conditions. This information was used to calibrate the signal intensity of a variety of oligonucleotide probes spotted onto the microarrays for the detection of toxic algae (MIDTAL), which is being developed to aid national monitoring agencies and to provide a faster means of identifying and quantifying harmful phytoplankton in water column samples.
dc.publisherOxford University Press (OUP)
dc.relation.ispartofFEMS Microbiology Ecology
dc.subjectmicroflagellates
dc.subjectrna
dc.subjectcalibration curves
dc.subjectmicroarrays
dc.subjectmidtal
dc.subjectreal-time pcr
dc.subjectirish coastal waters
dc.subjectharmful algal blooms
dc.subjecttoxic algae
dc.subjectoligonucleotide microarray
dc.subjectnatural-populations
dc.subjectbacterial pathogens
dc.subjectsequence data
dc.subjectprobes
dc.subjecthaptophyta
dc.titleAn assessment of rna content inprymnesium parvum,prymnesium polylepis,cf.chattonellasp. andkarlodinium veneficumunder varying environmental conditions for calibrating an rna microarray for species detection
dc.typeArticle
dc.identifier.doi10.1111/1574-6941.12277
dc.local.publishedsourcehttps://academic.oup.com/femsec/article-pdf/88/1/140/19538399/88-1-140.pdf
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