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dc.contributor.authorMani, Vigneshwaran
dc.contributor.authorWasalathanthri, Dhanuka P.
dc.contributor.authorJoshi, Amit A.
dc.contributor.authorKumar, Challa V.
dc.contributor.authorRusling, James F.
dc.date.accessioned2018-09-20T16:15:51Z
dc.date.available2018-09-20T16:15:51Z
dc.date.issued2012-12-04
dc.identifier.citationMani, Vigneshwaran; Wasalathanthri, Dhanuka P. Joshi, Amit A.; Kumar, Challa V.; Rusling, James F. (2012). Highly efficient binding of paramagnetic beads bioconjugated with 100 000 or more antibodies to protein-coated surfaces. Analytical Chemistry 84 (23), 10485-10491
dc.identifier.issn0003-2700,1520-6882
dc.identifier.urihttp://hdl.handle.net/10379/12626
dc.description.abstractWe report here the first kinetic characterization of 1 mu m diameter superparamagnetic particles (MP) decorated with over 100 000 antibodies binding to protein antigens attached to flat surfaces. Surface plasmon resonance (SPR) was used to show that these antibody-derivatized MPs (MP-Ab(2)) exhibit irreversible binding with 100-fold increased association rates compared to free antibodies. The estimated upper limit for the dissociation constant of MP-Ab2 from the SPR sensor surface is 5 fM, compared to 3-8 nM for the free antibodies. These results are explained by up to 2000 interactions of MP-Ab(2) with protein-decorated surfaces. Findings are consistent with highly efficient capture of protein antigens in solution by the MP-Ab(2) and explain in part the utility of these beads for ultrasensitive protein detection into the fM and aM range. Aggregation of these particles on the SPR chip, probably due to residual magnetic microdomains in the particles, also contributes to ultrasensitive detection and may also help drive the irreversible binding.
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofAnalytical Chemistry
dc.subjectultrasensitive detection
dc.subjectmultivalent interactions
dc.subjectgold nanoparticles
dc.subjectcancer biomarkers
dc.subjectbiosensor
dc.subjectaffinity
dc.subjecttechnologies
dc.subjectsimulation
dc.subjectstrategies
dc.subjectinterfaces
dc.titleHighly efficient binding of paramagnetic beads bioconjugated with 100 000 or more antibodies to protein-coated surfaces
dc.typeArticle
dc.identifier.doi10.1021/ac3028257
dc.local.publishedsourcehttp://europepmc.org/articles/pmc3514570?pdf=render
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