Colonisation with esbl-producing and carbapenemase-producing enterobacteriaceae, vancomycin-resistant enterococci, and meticillin-resistant staphylococcus aureus in a long-term care facility over one year
Johnson, James R
MetadataShow full item record
This item's downloads: 0 (view details)
Cited 39 times in Scopus (view citations)
Ludden, Catherine; Cormican, Martin; Vellinga, Akke; Johnson, James R; Austin, Bernie; Morris, Dearbháile (2015). Colonisation with esbl-producing and carbapenemase-producing enterobacteriaceae, vancomycin-resistant enterococci, and meticillin-resistant staphylococcus aureus in a long-term care facility over one year. BMC Infectious Diseases 15 ,
Background: This study examined colonisation with and characteristics of antimicrobial-resistant organisms among residents of a long-term care facility (LTCF) over one year, including strain persistence and molecular diversity among isolates of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae. Methods: Sixty-four residents of a LTCF were recruited (51 at baseline, 13 during the year). Data on dependency levels, hospitalisations, and antimicrobial prescribing were collected. Nasal and rectal swabs and catheter urine specimens were examined quarterly, using chromogenic agars, for ESBL-producing Enterobacteriaceae, carbapenemase-producing Enterobacteriaceae (CPE), vancomycin-resistant enterococci (VRE), and meticillin-resistant S. aureus (MRSA). All ESBL-producing E. coli (ESBL-EC) were characterised by pulsed-field gel electrophoresis (PFGE) and PCR to assess for sequence type (ST) ST131, its resistance-associated H30 and H30-Rx subclones, and bla(CTX-M), bla(TEM), bla(SHV), and bla(OXA-1). Results: The overall number of residents colonised, by organism, was as follows: ESBL-EC, 35 (55%); MRSA, 17 (27%); ESBL-producing K. pneumoniae (ESBL-KP), 5 (8%); VRE, 2 (3%) and CPE, 0 (0%). All 98 ESBL-EC isolates were H30-Rx ST131, with bla(CTX-M-group) 1. By PFGE, a group of 91 ESBL-EC (from 33 participants) had &gt;= 85% similar profiles and resembled UK epidemic strain A/ international pulsotype PFGE812. Sequential ESBL-EC from individual residents were closely related. Six ESBL-KP isolates, from five participants, had bla(CTX-M-group) 1 and by PFGE were closely related. Colonisation with ESBL and MRSA was associated with location within the LTCF and previous exposure to antimicrobials. Conclusions: Among LTCF residents, colonisation with ESBL-EC and MRSA was common. All ESBL-EC were H30-Rx ST131, consistent with clonal dissemination.
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland
Showing items related by title, author, creator and subject.
Effect of subinhibitory concentrations of benzalkonium chloride on the competitiveness of pseudomonas aeruginosa grown in continuous culture Mc Cay, P. H.; Ocampo-Sosa, A. A.; Fleming, G. T. A. (Microbiology Society, 2009-10-08)This study investigates the link between adaptation to biocides and antibiotics in Pseudomonas aeruginosa. An enrichment continuous culture of P. aeruginosa NCIMB 10421 (MIC 25 mg BKC l(-1)) was operated (D=0.04 h(-1), 792 ...
Epidemiological cut-off values forflavobacterium psychrophilummic data generated by a standard test protocol Smith, P; Endris, R; Kronvall, G; Thomas, V; Verner-Jeffreys, D; Wilhelm, C; Dalsgaard, I (Wiley-Blackwell, 2014-12-26)Epidemiological cut-off values were developed for application to antibiotic susceptibility data for Flavobacterium psychrophilum generated by standard CLSI test protocols. The MIC values for ten antibiotic agents against ...
SMITH, P.G. (O.I.E (World Organisation for Animal Health), 2008-04-01)Appropriate antimicrobial therapy represents one of the most effective management responses to emergencies associated with infectious disease epizootics. The use of these agents, however, has the potential to increase the ...