Specific ion effects on macromolecular interactions inescherichia coliextracts

Abstract

Protein characterization in situ remains a major challenge for protein science. Here, the interactions of Tat-GB1 in Escherichia coli cell extracts were investigated by NMR spectroscopy and size exclusion chromatography (SEC). Tat-GB1 was found to participate in high molecular weight complexes that remain intact at physiologically-relevant ionic strength. This observation helps to explain why Tat-GB1 was not detected by in-cell NMR spectroscopy. Extracts pre-treated with RNase A had a different SEC elution profile indicating that Tat-GB1 predominantly interacted with RNA. The roles of biological and laboratory ions in mediating macromolecular interactions were studied. Interestingly, the interactions of Tat-GB1 could be disrupted by biologically-relevant multivalent ions. The most effective shielding of interactions occurred in Mg2+-containing buffers. Moreover, a combination of RNA digestion and Mg2+ greatly enhanced the NMR detection of Tat-GB1 in cell extracts.