Nucleolar activity and cenp-c regulate cenp-a and cal1 availability for centromere assembly in meiosis
Collins, Caitriona M.
Dattoli, Anna A.
Dunleavy, Elaine M.
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Kwenda, Lucretia; Collins, Caitriona M. Dattoli, Anna A.; Dunleavy, Elaine M. (2016). Nucleolar activity and cenp-c regulate cenp-a and cal1 availability for centromere assembly in meiosis. Development 143 (8), 1400-1412
The centromere-specific histone CENP-A is the key epigenetic determinant of centromere identity. Whereas most histones are removed from mature sperm, CENP-A is retained to mark paternal centromeres. In Drosophila males we show that the centromere assembly factors CAL1 and CENP-C are required for meiotic chromosome segregation, CENP-A assembly and maintenance on sperm, as well as fertility. In meiosis, CENP-A accumulates with CAL1 in nucleoli. Furthermore, we show that CENP-C normally limits the release of CAL1 and CENP-A from nucleoli for proper centromere assembly in meiotic prophase I. Finally, we show that RNA polymerase I transcription is required for efficient CENP-A assembly in meiosis, as well as centromere tethering to nucleoli.