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dc.contributor.authorKadimisetty, Karteek
dc.contributor.authorMalla, Spundana
dc.contributor.authorSardesai, Naimish P.
dc.contributor.authorJoshi, Amit A.
dc.contributor.authorFaria, Ronaldo C.
dc.contributor.authorLee, Norman H.
dc.contributor.authorRusling, James F.
dc.date.accessioned2018-09-20T16:12:22Z
dc.date.available2018-09-20T16:12:22Z
dc.date.issued2015-04-21
dc.identifier.citationKadimisetty, Karteek; Malla, Spundana; Sardesai, Naimish P. Joshi, Amit A.; Faria, Ronaldo C.; Lee, Norman H.; Rusling, James F. (2015). Automated multiplexed ecl immunoarrays for cancer biomarker proteins. Analytical Chemistry 87 (8), 4472-4478
dc.identifier.issn0003-2700,1520-6882
dc.identifier.urihttp://hdl.handle.net/10379/12133
dc.description.abstractPoint-of-care diagnostics based on multiplexed protein measurements face challenges of simple, automated, low-cost, and high-throughput operation-with high sensitivity. Herein, we describe an automated, microprocessor-controlled microfluidic immunoarray for simultaneous multiplexed detection of small protein panels in complex samples. A microfluidic sample/reagent delivery cassette was coupled to a 30-microwell detection array to achieve sensitive detection of four prostate cancer biomarker proteins in serum. The proteins are prostate specific antigen (PSA), prostate specific membrane antigen (PSMA), platelet factor-4 (PF-4), and interlukin-6 (IL-6). The six channel system is driven by integrated mitropumps controlled by an inexpensive programmable microprocessor. The reagent delivery cassette and detection array feature channels made by precision-cut 0.8 mm silicone gaskets. Single-wall carbon nanotube forests were grown in printed microwells on a pyrolytic graphite detection chip and decorated with capture antibodies. The detection chip is housed in a machined microfluidic chamber with a steel metal shim counter electrode and Ag/AgCl reference electrode for electrochemiluminescent (ECL) measurements. The preloaded sample/reagent cassette automatically delivers samples, wash buffers, and ECL RuBPY-silica antibody detection nanoparticles sequentially. An onboard microcontroller controls micropumps, and reagent flow to the detection chamber according to a preset program. Detection employs tripropylamine, a sacrificial reductant, while applying 0.95 V vs Ag/AgCl. Resulting ECL light was measured by a CCD camera. Ultra low detection limits of 10-100 fg mL(-1) were achieved in simultaneous detection of the four protein in 36 min assays. Results for the four proteins in prostate cancer patient serum gave excellent correlation with those from single-protein ELISA.
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofAnalytical Chemistry
dc.subjectelectrogenerated chemiluminescence
dc.subjectultrasensitive detection
dc.subjectmicrofluidic devices
dc.subjectprostate-cancer
dc.subjectamplification
dc.subjectdiagnostics
dc.subjectarrays
dc.subjectproteome
dc.subjectvalves
dc.subjectserum
dc.titleAutomated multiplexed ecl immunoarrays for cancer biomarker proteins
dc.typeArticle
dc.identifier.doi10.1021/acs.analchem.5b00421
dc.local.publishedsourcehttp://europepmc.org/articles/pmc4437514?pdf=render
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