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dc.contributor.authorGrob, A.
dc.contributor.authorColleran, C.
dc.contributor.authorMcStay, B.
dc.date.accessioned2018-09-20T16:09:44Z
dc.date.available2018-09-20T16:09:44Z
dc.date.issued2014-01-21
dc.identifier.citationGrob, A. Colleran, C.; McStay, B. (2014). Construction of synthetic nucleoli in human cells reveals how a major functional nuclear domain is formed and propagated through cell division. Genes & Development 28 (3), 220-230
dc.identifier.issn0890-9369
dc.identifier.urihttp://hdl.handle.net/10379/11737
dc.description.abstractHuman cell nuclei are functionally organized into structurally stable yet dynamic bodies whose cell cycle inheritance is poorly understood. Here, we investigate the biogenesis and propagation of nucleoli, sites of ribosome biogenesis and key regulators of cellular growth. Nucleolar and cell cycles are intimately connected. Nucleoli disappear during mitosis, reforming around prominent uncharacterized chromosomal features, nucleolar organizer regions (NORs). By examining the effects of UBF depletion on both endogenous NORs and synthetic pseudo-NORs, we reveal its essential role in maintaining competency and establishing a bookmark on mitotic NORs. Furthermore, we demonstrate that neo-NORs, UBF-binding site arrays coupled with rDNA transcription units, direct the de novo biogenesis of functional compartmentalized neonucleoli irrespective of their site of chromosomal integration. For the first time, we establish the sequence requirements for nucleolar biogenesis and provide proof that this is a staged process where UBF-dependent mitotic bookmarking precedes functiondependent nucleolar assembly.
dc.publisherCold Spring Harbor Laboratory
dc.relation.ispartofGenes & Development
dc.subjectnucleolus
dc.subjectnucleolar organizer region (nor)
dc.subjectubf
dc.subjectmitotic bookmarking
dc.subjectpseudo-nor
dc.subjectneo-nor
dc.subjectribosomal-rna gene
dc.subjectorganizer regions
dc.subjectpolymerase-i
dc.subjectgenomic architecture
dc.subjecttranscription
dc.subjectubf
dc.subjectDNA
dc.subjectmaintenance
dc.subjectnors
dc.subjectchromosomes
dc.titleConstruction of synthetic nucleoli in human cells reveals how a major functional nuclear domain is formed and propagated through cell division
dc.typeArticle
dc.identifier.doi10.1101/gad.234591.113
dc.local.publishedsourcehttp://genesdev.cshlp.org/content/28/3/220.full.pdf
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