Search
Now showing items 1-6 of 6
Monitoring local unfolding of bovine serum albumin during denaturation using steady-state and time-resolved fluorescence spectroscopy
(Springer, 2010-03)
In a previous report (J. Fluoresc. 16, 153, 2006) we studied the chaotropiclly induced denaturation of Bovine Serum Albumin (BSA) using the fluorescence decay kinetics at different stages in the denaturation of BSA by ...
A fluorescence anisotropy method for measuring protein concentration in complex cell culture media
(Elsevier, 2014-03-12)
The rapid, quantitative analysis of the complex cell culture media used in biopharmaceutical manufacturing is of critical importance. Requirements for cell culture media composition profiling, or changes in specific analyte ...
Investigating native state fluorescence emission of Immunoglobulin G using polarized Excitation Emission Matrix (pEEM) spectroscopy and PARAFAC
(Elsevier, 2018-12-20)
Intrinsic fluorescence spectroscopy (IFS) measurements for protein structural analysis can be enhanced by the use of anisotropy resolved multidimensional emission spectroscopy (ARMES). ARMES attempts to overcome the ...
Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy
(Elsevier, 2019-12-10)
Immunoglobulin G (IgG) is often used as a starting material for the production of functionalised antibodies, like Antibody Drug Conjugates (ADCs), PEGlyated-conjugates, or radioimmunoconjugates. The gross structural quality ...
Characterization of Lysozyme PEGylation products using polarized Excitation Emission Matrix (pEEM) spectroscopy
(Wiley, 2020-07-07)
The growing use of therapeutic proteins requires accurate measurement techniques for measuring biophysical and structural changes during manufacturing. This is particularly true for PEGylation of proteins, because ...
Modelling Förster resonance energy transfer (FRET) using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
(Elsevier, 2020-11-16)
Background
Förster Resonance Energy Transfer (FRET) is widely used to study the structure and dynamics of biomolecular systems and also causes the non-linear fluorescence response observed in multi-fluorophore proteins. ...