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Prediction of Cell Culture Media Performance Using Fluorescence Spectroscopy
(2010)
Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ...
Rapid quantification of tryptophan and tyrosine in chemically defined cell culture media using fluorescence spectroscopy
(2012)
The rapid and inexpensive analysis of the complex cell culture media used in industrial mammalian cell culture is required for quality and variance monitoring. Excitation-emission matrix (EEM) spectroscopy combined with ...
Monitoring local unfolding of bovine serum albumin during denaturation using steady-state and time-resolved fluorescence spectroscopy
(Springer, 2010-03)
In a previous report (J. Fluoresc. 16, 153, 2006) we studied the chaotropiclly induced denaturation of Bovine Serum Albumin (BSA) using the fluorescence decay kinetics at different stages in the denaturation of BSA by ...
Super stable fluorescein isothiocyanate isomer I monolayer for total internal reflection fluorescence microscopy
(American Chemical Society, 2018-08-27)
Total internal reflection fluorescence microscopy (TIRFM) is an important method in surface science and for the analysis of surface-bound macromolecules. Here, we developed and explored the use of a novel fluorescein ...
Study of water adsorption in PolyN-isopropylacrylamide) thin films using fluorescence emission of 3-hydroxyflavone probes
(ACS Publications, 2010)
The noncontact measurement of water uptake in microscale , thermoresponsive poly(N-isopropylacrylamide) thin films is challenging. We assessed the efficacy of three different 3-hydroxyflavone (3-HF)-based fluorophores to ...
Prediction of Cell Culture Media performance using Fluorescence Spectroscopy
(ACS Publications, 2010)
Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ...
Prediction of cell culture media performance using fluorescence spectroscopy.
(American Chemical Society, 2010)
Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ...
A fluorescence anisotropy method for measuring protein concentration in complex cell culture media
(Elsevier, 2014-03-12)
The rapid, quantitative analysis of the complex cell culture media used in biopharmaceutical manufacturing is of critical importance. Requirements for cell culture media composition profiling, or changes in specific analyte ...
Investigating native state fluorescence emission of Immunoglobulin G using polarized Excitation Emission Matrix (pEEM) spectroscopy and PARAFAC
(Elsevier, 2018-12-20)
Intrinsic fluorescence spectroscopy (IFS) measurements for protein structural analysis can be enhanced by the use of anisotropy resolved multidimensional emission spectroscopy (ARMES). ARMES attempts to overcome the ...
Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy
(Elsevier, 2019-12-10)
Immunoglobulin G (IgG) is often used as a starting material for the production of functionalised antibodies, like Antibody Drug Conjugates (ADCs), PEGlyated-conjugates, or radioimmunoconjugates. The gross structural quality ...