Browsing School of Chemistry (Scholarly Articles) by Author "Calvet, Amandine"
Now showing items 1-8 of 8
-
Chemometric approaches to low-content quantification (LCQ) in solid-state mixtures using Raman mapping spectroscopy.
Li, Boyan; Casamayou-Boucau, Yannick; Calvet, Amandine; Ryder, Alan G. (Royal Society of Chemistry, 2017-10-30)The low-content quantification (LCQ) of active pharmaceutical ingredients or impurities in solid mixtures is important in pharmaceutical manufacturing and analysis. We previously demonstrated the feasibility of using Raman ... -
Comprehensive, quantitative bioprocess productivity monitoring using fluorescence EEM spectroscopy and chemometrics
Calvet, Amandine; Ryder, Alan G. (Royal Society of Chemistry, 2014)This study demonstrates the application of fluorescence excitation-emission matrix (EEM) spectroscopy to the quantitative predictive analysis of recombinant glycoprotein production cultured in a Chinese hamster ovary (CHO) ... -
A fluorescence anisotropy method for measuring protein concentration in complex cell culture media
Groza, RC,Calvet, A,Ryder, AG; Groza, Radu Constantin; Calvet, Amandine; Ryder, Alan G. (Elsevier, 2014-03-12)The rapid, quantitative analysis of the complex cell culture media used in biopharmaceutical manufacturing is of critical importance. Requirements for cell culture media composition profiling, or changes in specific analyte ... -
Investigating tryptophan quenching of fluorescein fluorescence under protolytic equilibrium
Ryder, Alan G.; Szczupak, Boguslaw; Togashi, Denisio M.; Calvet, Amandine; O'Loughlin, Muireann (2009)Fluorescein is one of most used fluorescent labels for characterizing biological systems, such as proteins, and is used in fluorescence microscopy. However, if fluorescein is to be used for quantitative measurements involving ... -
Kernel principal component analysis residual diagnosis (KPCARD): an automated method to remove cosmic ray artefacts in Raman spectra.
Li, Boyan; Calvet, Amandine; Casamayou-Boucau, Yannick; Ryder, Alan G. (2016)A new, fully automated, rapid method, referred to as kernel principal component analysis residual diagnosis (KPCARD), is proposed for removing cosmic ray artifacts (CRAs) in Raman spectra, and in particular for large Raman ... -
Low-content quantification in powders using Raman spectroscopy: a facile chemometric approach to sub 0.1% limits of detection.
Li, Boyan; Calvet, Amandine; Ryder, Alan G.; Casamayou-Boucau, Yannick; Morris, Cheryl (American Chemical Society, 2015-02-23)A robust and accurate analytical methodology for low-content (<0.1%) quantification in the solid-state using Raman spectroscopy, sub-sampling, and chemometrics was demonstrated using a piracetam–proline model. The method ... -
Monitoring cell culture media degradation using surface enhanced Raman scattering (SERS) spectroscopy
Calvet, Amandine; Ryder, Alan G. (Elsevier (Science Direct), 2014-06-13)The quality of the cell culture media used in biopharmaceutical manufacturing is a crucial factor affecting bioprocess performance and the quality of the final product. Due to their complex composition these media are ... -
Rapid quantification of tryptophan and tyrosine in chemically defined cell culture media using fluorescence spectroscopy
Ryder, Alan G.; Calvet, Amandine; Li, Boyan (2012)The rapid and inexpensive analysis of the complex cell culture media used in industrial mammalian cell culture is required for quality and variance monitoring. Excitation-emission matrix (EEM) spectroscopy combined with ...