dc.contributor.author | Fenwick, M A | |
dc.contributor.author | Llewellyn, S | |
dc.contributor.author | Fitzpatrick, R | |
dc.contributor.author | Kenny, D A | |
dc.contributor.author | Murphy, J J | |
dc.contributor.author | Patton, J | |
dc.contributor.author | Wathes, D C | |
dc.date.accessioned | 2018-09-20T16:07:55Z | |
dc.date.available | 2018-09-20T16:07:55Z | |
dc.date.issued | 2007-12-25 | |
dc.identifier.citation | Fenwick, M A; Llewellyn, S; Fitzpatrick, R; Kenny, D A; Murphy, J J; Patton, J; Wathes, D C (2007). Negative energy balance in dairy cows is associated with specific changes in igf-binding protein expression in the oviduct. Reproduction 135 (1), 63-75 | |
dc.identifier.issn | 1470-1626,1741-7899 | |
dc.identifier.uri | http://hdl.handle.net/10379/11450 | |
dc.description.abstract | Negative energy balance (NEB) during early lactation in dairy cows leads to an altered metabolic state that has major effects on the production of IGF family members. Low IGF-I concentrations are associated with poor fertility and therefore we aimed to determine whether NEB exerts a direct effect on IGF expression in the postpartum oviduct. Multiparous Holstein cows were allocated to two treatments (each n=6) designed using differential feeding and milking regimes to produce either mild NEB (MNEB) or severe NEB (SNEB). Animals were slaughtered in week 2 of lactation when divergent metabolic profiles were evident. Oviducts were collected for RNA analysis by real-time RT-PCR and in situ hybridisation. Quantitative measures in oviduct gene expression were obtained for all members of the IGF family (IGF-I/II, IGF-binding proteins (IGFBP) 1-6 and receptors for IGF types 1 and 2), insulin A/B, GH, glucocorticoid and oestrogen alpha/beta. Expression of IGFBP-2 and IGFBP-6 (both of which have a high affinity for IGF-II) was decreased in SNEB relative to MNEB (P<0.05). No other gene was altered by NEB, but IGF-II, IGFBP-3, IGFBP-5 and IGFBP-6 all showed differential expression in different regions of the oviduct. These results indicate that, in addition to low circulating IGF-I after calving, NEB may also influence IGF availability in the oviduct indirectly through changes in specific IGFBP expression. It is possible that the predicted increased signalling by IGF-II may perturb embryo development, contributing to the high rates of embryonic mortality in dairy cows. | |
dc.publisher | BioScientifica | |
dc.relation.ispartof | Reproduction | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | growth-factor-i | |
dc.subject | messenger-rna expression | |
dc.subject | insulin-like | |
dc.subject | estrous-cycle | |
dc.subject | bovine oviduct | |
dc.subject | fallopian-tube | |
dc.subject | early-pregnancy | |
dc.subject | vitro culture | |
dc.subject | morphological-changes | |
dc.subject | biological actions | |
dc.title | Negative energy balance in dairy cows is associated with specific changes in igf-binding protein expression in the oviduct | |
dc.type | Article | |
dc.identifier.doi | 10.1530/rep-07-0243 | |
dc.local.publishedsource | http://www.reproduction-online.org/content/135/1/63.full.pdf | |
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