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dc.contributor.authorEykelenboom, John Kenneth
dc.contributor.authorHarte, Emma Christina
dc.contributor.authorCanavan, Lynn
dc.contributor.authorPastor-Peidro, Ana
dc.contributor.authorCalvo-Asensio, Irene
dc.contributor.authorLlorens-Agost, Marta
dc.contributor.authorLowndes, Noel Francis
dc.date.accessioned2018-09-20T16:07:26Z
dc.date.available2018-09-20T16:07:26Z
dc.date.issued2013-11-01
dc.identifier.citationEykelenboom, John Kenneth; Harte, Emma Christina; Canavan, Lynn; Pastor-Peidro, Ana; Calvo-Asensio, Irene; Llorens-Agost, Marta; Lowndes, Noel Francis (2013). Atr activates the s-m checkpoint during unperturbed growth to ensure sufficient replication prior to mitotic onset. Cell Reports 5 (4), 1095-1107
dc.identifier.issn2211-1247
dc.identifier.urihttp://hdl.handle.net/10379/11382
dc.description.abstractCells must accurately replicate and segregate their DNA once per cell cycle in order to successfully transmit genetic information. During S phase in the presence of agents that cause replication stress, ATR-dependent checkpoints regulate origin firing and the replication machinery as well as prevent untimely mitosis. Here, we investigate the role of ATR during unperturbed growth in vertebrate cells. In the absence of ATR, individual replication forks progress more slowly, and an increased number of replication origins are activated. These cells also enter mitosis early and divide more rapidly, culminating in chromosome bridges and laggards at anaphase, failed cytokinesis, and cell death. Interestingly, cell death can be rescued by prolonging mitosis with partial inhibition of the mitotic cyclin-dependent kinase 1. Our data indicate that one of the essential roles of ATR during normal growth is to minimize the level of unreplicated DNA before the onset of mitosis.
dc.publisherElsevier BV
dc.relation.ispartofCell Reports
dc.subjectcyclin-dependent kinases
dc.subjectDNA-replication
dc.subjectbudding yeast
dc.subjectchromosome-replication
dc.subjectfork progression
dc.subjectphase checkpoint
dc.subjectsaccharomyces-cerevisiae
dc.subjectcentrosome amplification
dc.subjectcdk inhibitors
dc.subjectfragile sites
dc.titleAtr activates the s-m checkpoint during unperturbed growth to ensure sufficient replication prior to mitotic onset
dc.typeArticle
dc.identifier.doi10.1016/j.celrep.2013.10.027
dc.local.publishedsourcehttps://doi.org/10.1016/j.celrep.2013.10.027
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