Trail receptor gene editing unveils trail-r1 as a master player of apoptosis induced by trail and er stres
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2016-12-27Author
Florent, Dufour
Thibault, Rattier
Andrei Alexandru, Constantinescu
Luciana, Zischler
Aymeric, Morlé
Hazem, Ben Mabrouk
Etienne, Humblin
Guillaume, Jacquemin
Eva, Szegezdi
Fabien, Delacote
Naziha, Marrakchi
Gilles, Guichard
Catherine, Pellat-Deceunyn
Pierre, Vacher
Patrick, Legembre
Carmen, Garrido
Micheau, Olivier
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Florent, Dufour; Thibault, Rattier; Andrei Alexandru, Constantinescu; Luciana, Zischler; Aymeric, Morlé; Hazem, Ben Mabrouk; Etienne, Humblin; Guillaume, Jacquemin; Eva, Szegezdi; Fabien, Delacote; Naziha, Marrakchi; Gilles, Guichard; Catherine, Pellat-Deceunyn; Pierre, Vacher; Patrick, Legembre; Carmen, Garrido; Micheau, Olivier (2016). Trail receptor gene editing unveils trail-r1 as a master player of apoptosis induced by trail and er stres. Oncotarget 8 (6), 9974-9985
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Abstract
TRAIL induces selective tumor cell death through TRAIL-R1 and TRAIL-R2. Despite the fact that these receptors share high structural homologies, induction of apoptosis upon ER stress, cell autonomous motility and invasion have solely been described to occur through TRAIL-R2. Using the TALEN gene-editing approach, we show that TRAIL-R1 can also induce apoptosis during unresolved unfolded protein response (UPR). Likewise, TRAIL-R1 was found to co-immunoprecipitate with FADD and caspase-8 during ER stress. Its deficiency conferred resistance to apoptosis induced by thaspigargin, tunicamycin or brefeldin A. Our data also demonstrate that tumor cell motility and invasion-induced by TRAIL-R2 is not cell autonomous but induced in a TRAIL-dependant manner. TRAIL-R1, on the other hand, is unable to trigger cell migration owing to its inability to induce an increase in calcium flux. Importantly, all the isogenic cell lines generated in this study revealed that apoptosis induced TRAIL is preferentially induced by TRAIL-R1. Taken together, our results provide novel insights into the physiological functions of TRAIL-R1 and TRAIL-R2 and suggest that targeting TRAIL-R1 for anticancer therapy is likely to be more appropriate owing to its lack of pro-motile signaling capability.