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dc.contributor.authorDornblut, C.
dc.contributor.authorQuinn, N.
dc.contributor.authorMonajambashi, S.
dc.contributor.authorPrendergast, L.
dc.contributor.authorvan Vuuren, C.
dc.contributor.authorMunch, S.
dc.contributor.authorDeng, W.
dc.contributor.authorLeonhardt, H.
dc.contributor.authorCardoso, M. C.
dc.contributor.authorHoischen, C.
dc.contributor.authorDiekmann, S.
dc.contributor.authorSullivan, K. F.
dc.identifier.citationDornblut, C. Quinn, N.; Monajambashi, S.; Prendergast, L.; van Vuuren, C.; Munch, S.; Deng, W.; Leonhardt, H.; Cardoso, M. C.; Hoischen, C.; Diekmann, S.; Sullivan, K. F. (2014). A cenp-s/x complex assembles at the centromere in s and g2 phases of the human cell cycle. Open Biology 4 (2),
dc.description.abstractThe functional identity of centromeres arises from a set of specific nucleoprotein particle subunits of the centromeric chromatin fibre. These include CENP-A and histone H3 nucleosomes and a novel nucleosome-like complex of CENPs -T, -W, -S and -X. Fluorescence cross-correlation spectroscopy and Forster resonance energy transfer (FRET) revealed that human CENP-S and -X exist principally in complex in soluble form and retain proximity when assembled at centromeres. Conditional labelling experiments show that they both assemble de novo during S phase and G2, increasing approximately three-to fourfold in abundance at centromeres. Fluorescence recovery after photobleaching (FRAP) measurements documented steady-state exchange between soluble and assembled pools, with CENP-X exchanging approximately 10 times faster than CENP-S (t(1/2) similar to 10 min versus 120 min). CENP-S binding to sites of DNA damage was quite distinct, with a FRAP half-time of approximately 160 s. Fluorescent two-hybrid analysis identified CENP-T as a uniquely strong CENP-S binding protein and this association was confirmed by FRET, revealing a centromere-bound complex containing CENP-S, CENP-X and CENP-T in proximity to histone H3 but not CENP-A. We propose that deposition of the CENP-T/W/S/X particle reveals a kinetochore-specific chromatin assembly pathway that functions to switch centromeric chromatin to a mitosis-competent state after DNA replication. Centromeres shuttle between CENP-A-rich, replication-competent and H3-CENP-T/W/S/X-rich mitosis-competent compositions in the cell cycle.
dc.publisherThe Royal Society
dc.relation.ispartofOpen Biology
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjectconstitutive centromere-associated network
dc.subjectliving human-cells
dc.subjecta nucleosomes
dc.subjectouter kinetochore
dc.subjectndc80 complex
dc.subjecthistone h3
dc.titleA cenp-s/x complex assembles at the centromere in s and g2 phases of the human cell cycle

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