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dc.contributor.authorDeepak, Vishwa
dc.contributor.authorKayastha, Pushpalata
dc.contributor.authorMcNamara, Laoise M.
dc.date.accessioned2018-09-20T16:05:35Z
dc.date.available2018-09-20T16:05:35Z
dc.date.issued2017-03-31
dc.identifier.citationDeepak, Vishwa; Kayastha, Pushpalata; McNamara, Laoise M. (2017). estrogen deficiency attenuates fluid flow–induced [ca 2+ ] i oscillations and mechanoresponsiveness of mlo-y4 osteocytes . The FASEB Journal 31 (7), 3027-3039
dc.identifier.issn0892-6638,1530-6860
dc.identifier.urihttp://hdl.handle.net/10379/11123
dc.description.abstractIt has been proposed that estrogen regulates the mechanosensitivity of osteocytes; however, the effects of estrogen deficiency that arises during postmenopausal osteoporosis on mechanical stimulation induced calcium signaling in osteocytes remain elusive. Here, we pretreated MLO-Y4 osteocytes with 10 nM E2 for 2, 3 and 5 d, then simulated postmenopausal conditions either by estrogen withdrawal (EW) from culture medium, or by inhibiting the estrogen receptor by using fulvestrant and estrogen (FE; ICI 182,780) in vitro. We investigated [Ca2+](i) oscillations and mechanobiologic responses of osteocytes (EW and FE) that were exposed to oscillatory fluid flow (OFF; 1 Pa, 0.5 Hz). We demonstrated that estrogen treatment enhanced OFF-induced [Ca2+](i) oscillations and that this effect was abrogated both by FE and EW. Moreover, osteocytes in both estrogen-depleted groups (EW and FE) had reduced levels of NO and prostaglandin E2 release, down-regulated dentin matrix protein-1, sclerostin, osteopontin, osteocalcin, and alkaline phosphatase mRNA expression, and reduced F-actin fiber formation after OFF stimulation compared with estrogen-treated cells. We propose a link between estrogen deficiency and alterations in [Ca2+](i)-mediated mechanosensitivity of osteocytes, which ultimately alter osteocyte function and differentiation. Deepak, V., Kayastha, P., McNamara, L. M. Estrogen deficiency attenuates fluid flow induced [Ca2+](i) oscillations and mechanoresponsiveness of MLO-Y4 osteocytes.
dc.publisherFASEB
dc.relation.ispartofThe FASEB Journal
dc.subjecte-2
dc.subjectmechanobiology
dc.subjectfluid shear stress
dc.subjectosteoporosis
dc.subjectmenopause
dc.subjectdentin matrix protein-1
dc.subjectserum sclerostin levels
dc.subjectstress in-vitro
dc.subjectbone-cells
dc.subjectnitric-oxide
dc.subjectmechanical strain
dc.subjectreceptor-alpha
dc.subjectpostmenopausal women
dc.subjectgene-expression
dc.subjectosteoblasts
dc.titleestrogen deficiency attenuates fluid flow–induced [ca 2+ ] i oscillations and mechanoresponsiveness of mlo-y4 osteocytes
dc.typeArticle
dc.identifier.doi10.1096/fj.201601280r
dc.local.publishedsourcehttp://www.fasebj.org/doi/pdf/10.1096/fj.201601280R
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