estrogen deficiency attenuates fluid flow–induced [ca 2+ ] i oscillations and mechanoresponsiveness of mlo-y4 osteocytes
dc.contributor.author | Deepak, Vishwa | |
dc.contributor.author | Kayastha, Pushpalata | |
dc.contributor.author | McNamara, Laoise M. | |
dc.date.accessioned | 2018-09-20T16:05:35Z | |
dc.date.available | 2018-09-20T16:05:35Z | |
dc.date.issued | 2017-03-31 | |
dc.identifier.citation | Deepak, Vishwa; Kayastha, Pushpalata; McNamara, Laoise M. (2017). estrogen deficiency attenuates fluid flow–induced [ca 2+ ] i oscillations and mechanoresponsiveness of mlo-y4 osteocytes . The FASEB Journal 31 (7), 3027-3039 | |
dc.identifier.issn | 0892-6638,1530-6860 | |
dc.identifier.uri | http://hdl.handle.net/10379/11123 | |
dc.description.abstract | It has been proposed that estrogen regulates the mechanosensitivity of osteocytes; however, the effects of estrogen deficiency that arises during postmenopausal osteoporosis on mechanical stimulation induced calcium signaling in osteocytes remain elusive. Here, we pretreated MLO-Y4 osteocytes with 10 nM E2 for 2, 3 and 5 d, then simulated postmenopausal conditions either by estrogen withdrawal (EW) from culture medium, or by inhibiting the estrogen receptor by using fulvestrant and estrogen (FE; ICI 182,780) in vitro. We investigated [Ca2+](i) oscillations and mechanobiologic responses of osteocytes (EW and FE) that were exposed to oscillatory fluid flow (OFF; 1 Pa, 0.5 Hz). We demonstrated that estrogen treatment enhanced OFF-induced [Ca2+](i) oscillations and that this effect was abrogated both by FE and EW. Moreover, osteocytes in both estrogen-depleted groups (EW and FE) had reduced levels of NO and prostaglandin E2 release, down-regulated dentin matrix protein-1, sclerostin, osteopontin, osteocalcin, and alkaline phosphatase mRNA expression, and reduced F-actin fiber formation after OFF stimulation compared with estrogen-treated cells. We propose a link between estrogen deficiency and alterations in [Ca2+](i)-mediated mechanosensitivity of osteocytes, which ultimately alter osteocyte function and differentiation. Deepak, V., Kayastha, P., McNamara, L. M. Estrogen deficiency attenuates fluid flow induced [Ca2+](i) oscillations and mechanoresponsiveness of MLO-Y4 osteocytes. | |
dc.publisher | FASEB | |
dc.relation.ispartof | The FASEB Journal | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | e-2 | |
dc.subject | mechanobiology | |
dc.subject | fluid shear stress | |
dc.subject | osteoporosis | |
dc.subject | menopause | |
dc.subject | dentin matrix protein-1 | |
dc.subject | serum sclerostin levels | |
dc.subject | stress in-vitro | |
dc.subject | bone-cells | |
dc.subject | nitric-oxide | |
dc.subject | mechanical strain | |
dc.subject | receptor-alpha | |
dc.subject | postmenopausal women | |
dc.subject | gene-expression | |
dc.subject | osteoblasts | |
dc.title | estrogen deficiency attenuates fluid flow–induced [ca 2+ ] i oscillations and mechanoresponsiveness of mlo-y4 osteocytes | |
dc.type | Article | |
dc.identifier.doi | 10.1096/fj.201601280r | |
dc.local.publishedsource | http://www.fasebj.org/doi/pdf/10.1096/fj.201601280R | |
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