Show simple item record

dc.contributor.authorde Vries-van Melle, ML
dc.contributor.author,
dc.contributor.authorTihaya, MS
dc.contributor.authorKops, N
dc.contributor.authorKoevoet, WJLM
dc.contributor.authorMurphy, JM
dc.contributor.authorVerhaar, JAN
dc.contributor.authorAlini, M
dc.contributor.authorEglin, D
dc.contributor.authorvan Osch, GJVM
dc.date.accessioned2018-09-20T16:05:27Z
dc.date.available2018-09-20T16:05:27Z
dc.date.issued2014-02-03
dc.identifier.citationde Vries-van Melle, ML; , ; Tihaya, MS; Kops, N; Koevoet, WJLM; Murphy, JM; Verhaar, JAN; Alini, M; Eglin, D; van Osch, GJVM (2014). Chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in a simulated osteochondral environment is hydrogel dependent. European Cells and Materials 27 , 112-123
dc.identifier.issn
dc.identifier.urihttp://hdl.handle.net/10379/11106
dc.description.abstractHydrogels pose interesting features for cartilage regeneration strategies, such as the option for injectability and in situ gelation resulting in optimal filling of defects. We aimed to study different hydrogels for their capability to support chondrogenesis of human bone marrow-derived mesenchymal stem cells (hBMSCs). hBMSCs were encapsulated in alginate, alginate with hyaluronic acid (alginate/HA), fibrin or thermoresponsive HA grafted with poly(N-isopropyl acrylamide) side-chains (HA-pNIPAM). Glycosaminoglycan production and cartilage-related gene expression were significantly higher in hBMSC-alginate and hBMSC-fibrin constructs than in the other constructs. Supplementation of alginate with HA was not beneficial. hBMSC-alginate, hBMSC-fibrin and hBMSC-HA-pNIPAM constructs were placed in simulated defects in osteochondral biopsies and cultured in vitro for 28 d. Biopsies containing hBMSC-alginate and hBMSC-fibrin were implanted subcutaneously in nude mice for 12 weeks. hBMSC-alginate constructs had significantly higher cartilage-related gene expression after 28 d of culture as well as significantly more safranin-O positive repair tissue after 12 weeks in vivo than hBMSC-fibrin constructs. Although initial experiments with hBMSC-hydrogel constructs suggested comparable results of hBMSC-alginate, hBMSC-fibrin and hBMSC-HA-pNIPAM constructs, culture in the osteochondral biopsy model in vitro as well as in vivo revealed differences, suggests that chondrogenesis of hBMSCs in an osteochondral environment is hydrogel-dependent.
dc.publisherECM Research Journal
dc.relation.ispartofEuropean Cells and Materials
dc.subjectcartilage tissue engineering
dc.subjectmesenchymal stem cells
dc.subjectalginate
dc.subjectfibrin
dc.subjecthyaluronic acid
dc.subjectarticular-cartilage repair
dc.subjectin-vitro chondrogenesis
dc.subjectfibrin glue
dc.subjecthyaluronic-acid
dc.subjectsulfated glycosaminoglycans
dc.subjectosteoarthritic cartilage
dc.subjectregenerative medicine
dc.subjectengineered cartilage
dc.subjecthuman chondrocytes
dc.subjectprogenitor cells
dc.titleChondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in a simulated osteochondral environment is hydrogel dependent
dc.typeArticle
dc.identifier.doi10.22203/ecm.v027a09
dc.local.publishedsourcehttp://doi.org/10.22203/ecm.v027a09
nui.item.downloads0


Files in this item

This item appears in the following Collection(s)

Show simple item record