• Login
    ARAN - Access to Research at NUI Galway
    View Item 
    •   ARAN Home
    • Support Services
    • Externally hosted open access publications with NUI Galway authors (2)
    • View Item
    •   ARAN Home
    • Support Services
    • Externally hosted open access publications with NUI Galway authors (2)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ARANCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypesThis CollectionBy Issue DateAuthorsTitlesSubjectsTypes

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Help

    How to submit and FAQs

    Role of subtyping in detecting salmonella cross contamination in the laboratory

    Thumbnail
    View/Open
    Full Text
    Date
    2009-01-01
    Author
    De Lappe, Niall
    Connor, Jean O
    Doran, Geraldine
    Devane, Genevieve
    Cormican, Martin
    Metadata
    Show full item record
    Usage
    This item's downloads: 0 (view details)
    
    Recommended Citation
    De Lappe, Niall; Connor, Jean O; Doran, Geraldine; Devane, Genevieve; Cormican, Martin (2009). Role of subtyping in detecting salmonella cross contamination in the laboratory. BMC Microbiology 9 ,
    Published Version
    https://bmcmicrobiol.biomedcentral.com/track/pdf/10.1186/1471-2180-9-155
    Abstract
    Background: With the exception of M. tuberculosis, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National Salmonella Reference Laboratory (Ireland) from 2000-2007 to identify likely incidents of laboratory cross contamination. Methods: Serotyping and antimicrobial susceptibility testing was performed on all Salmonella isolates received in the NSRL. Phage typing was performed on all S. Typhimurium and S. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected S. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars. Results: Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1). Conclusion: The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.
    URI
    http://hdl.handle.net/10379/11097
    Collections
    • Externally hosted open access publications with NUI Galway authors (2)
    • Copyright @ NUI Galway 2016
    • Library
    • NUI Galway