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dc.contributor.authorCunningham, Claire
dc.contributor.authorSrivastava, Akshay
dc.contributor.authorCollin, Estelle
dc.contributor.authorGrad, Sibylle
dc.contributor.authorAlini, Mauro
dc.contributor.authorPandit, Abhay
dc.contributor.authorWall, J. Gerard
dc.identifier.citationCunningham, Claire; Srivastava, Akshay; Collin, Estelle; Grad, Sibylle; Alini, Mauro; Pandit, Abhay; Wall, J. Gerard (2013). Isolation and characterisation of a recombinant antibody fragment that binds ncam1-expressing intervertebral disc cells. PLoS ONE 8 (12),
dc.description.abstractDegeneration of the intervertebral discs (IVD) is a leading cause of neck and low back pain. Degeneration begins in the central nucleus pulposus region, leading to loss of IVD osmotic properties. Regeneration approaches include administration of matrix-mimicking scaffolds, cells and/or therapeutic factors. Cell-targeting strategies are likely to improve delivery due to the low cell numbers in the IVD. Single-chain antibody fragments (scFvs) that bind IVD cells were isolated for potential delivery of therapeutics to degenerated IVD. The most cell-distal domain of neural cell adhesion molecule 1 (NCAM1) was cloned and expressed in Escherichia coli. Phage display technology was used to isolate a human scFv against the recombinant domain by panning a scFv library on the immobilised protein. The isolated scFv bound cultured rat astrocytes, as well as bovine nucleus pulposus and annulus fibrosus cells in immunocytochemical studies. The scFv also labelled cells in bovine spinal cord and six-month and two-year old bovine IVD sections by immunohistochemistry. Antibody fragments can provide cell-binding moieties at improved cost, time, yield and functionalisation potential over whole antibodies. The described scFv has potential application in delivery of therapeutics to NCAM1-expressing cells in degenerated IVD.
dc.publisherPublic Library of Science (PLoS)
dc.relation.ispartofPLoS ONE
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjecthuman nucleus pulposus
dc.subjectdomoic acid
dc.subjectadhesion molecule
dc.subjectannulus fibrosus
dc.subjectanulus fibrosus
dc.subjectphage display
dc.titleIsolation and characterisation of a recombinant antibody fragment that binds ncam1-expressing intervertebral disc cells

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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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