• Login
    ARAN - Access to Research at NUI Galway
    View Item 
    •   ARAN Home
    • Support Services
    • Externally hosted open access publications with NUI Galway authors (2)
    • View Item
    •   ARAN Home
    • Support Services
    • Externally hosted open access publications with NUI Galway authors (2)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ARANCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypesThis CollectionBy Issue DateAuthorsTitlesSubjectsTypes

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Help

    How to submit and FAQs

    Isolation and characterisation of a recombinant antibody fragment that binds ncam1-expressing intervertebral disc cells

    Thumbnail
    View/Open
    Full Text
    Date
    2013-12-13
    Author
    Cunningham, Claire
    Srivastava, Akshay
    Collin, Estelle
    Grad, Sibylle
    Alini, Mauro
    Pandit, Abhay
    Wall, J. Gerard
    Metadata
    Show full item record
    Usage
    This item's downloads: 0 (view details)
    Cited 7 times in Scopus (view citations)
    
    Recommended Citation
    Cunningham, Claire; Srivastava, Akshay; Collin, Estelle; Grad, Sibylle; Alini, Mauro; Pandit, Abhay; Wall, J. Gerard (2013). Isolation and characterisation of a recombinant antibody fragment that binds ncam1-expressing intervertebral disc cells. PLoS ONE 8 (12),
    Published Version
    https://doi.org/10.1371/journal.pone.0083678
    Abstract
    Degeneration of the intervertebral discs (IVD) is a leading cause of neck and low back pain. Degeneration begins in the central nucleus pulposus region, leading to loss of IVD osmotic properties. Regeneration approaches include administration of matrix-mimicking scaffolds, cells and/or therapeutic factors. Cell-targeting strategies are likely to improve delivery due to the low cell numbers in the IVD. Single-chain antibody fragments (scFvs) that bind IVD cells were isolated for potential delivery of therapeutics to degenerated IVD. The most cell-distal domain of neural cell adhesion molecule 1 (NCAM1) was cloned and expressed in Escherichia coli. Phage display technology was used to isolate a human scFv against the recombinant domain by panning a scFv library on the immobilised protein. The isolated scFv bound cultured rat astrocytes, as well as bovine nucleus pulposus and annulus fibrosus cells in immunocytochemical studies. The scFv also labelled cells in bovine spinal cord and six-month and two-year old bovine IVD sections by immunohistochemistry. Antibody fragments can provide cell-binding moieties at improved cost, time, yield and functionalisation potential over whole antibodies. The described scFv has potential application in delivery of therapeutics to NCAM1-expressing cells in degenerated IVD.
    URI
    http://hdl.handle.net/10379/10983
    Collections
    • Externally hosted open access publications with NUI Galway authors (2)
    • Copyright @ NUI Galway
    • Library
    • NUI Galway