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dc.contributor.authorConnelly, Stephanie
dc.contributor.authorShin, Seung G.
dc.contributor.authorDillon, Robert J.
dc.contributor.authorIjaz, Umer Z.
dc.contributor.authorQuince, Christopher
dc.contributor.authorSloan, William T.
dc.contributor.authorCollins, Gavin
dc.date.accessioned2018-09-20T16:03:52Z
dc.date.available2018-09-20T16:03:52Z
dc.date.issued2017-05-01
dc.identifier.citationConnelly, Stephanie; Shin, Seung G. Dillon, Robert J.; Ijaz, Umer Z.; Quince, Christopher; Sloan, William T.; Collins, Gavin (2017). Bioreactor scalability: laboratory-scale bioreactor design influences performance, ecology, and community physiology in expanded granular sludge bed bioreactors. Frontiers in Microbiology 8 ,
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10379/10873
dc.description.abstractStudies investigating the feasibility of new, or improved, biotechnologies, such as wastewater treatment digesters, inevitably start with laboratory-scale trials. However, it is rarely determined whether laboratory-scale results reflect full-scale performance or microbial ecology. The Expanded Granular Sludge Bed (EGSB) bioreactor, which is a high-rate anaerobic digester configuration, was used as a model to address that knowledge gap in this study. Two laboratory-scale idealizations of the EGSB-a one-dimensional and a three-dimensional scale-down of a full-scale design-were built and operated in triplicate under near-identical conditions to a full-scale EGSB. The laboratory-scale bioreactors were seeded using biomass obtained from the full-scale bioreactor, and, spent water from the distillation of whisky from maize was applied as substrate at both scales. Over 70 days, bioreactor performance, microbial ecology, and microbial community physiology were monitored at various depths in the sludge-beds using 16S rRNA gene sequencing (V4 region), specific methanogenic activity (SMA) assays, and a range of physical and chemicalmonitoringmethods. SMA assays indicated dominance of the hydrogenotrophic pathway at full-scale whilst a more balanced activity profile developed during the laboratory-scale trials. At each scale, Methanobacterium was the dominant methanogenic genus present. Bioreactor performance overall was better at laboratory-scale than full-scale. We observed that bioreactor design at laboratory-scale significantly influenced spatial distribution of microbial community physiology and taxonomy in the bioreactor sludge-bed, with 1-D bioreactor types promoting stratification of each. In the 1-D laboratory bioreactors, increased abundance of Firmicutes was associated with both granule position in the sludge bed and increased activity against acetate and ethanol as substrates. We further observed that stratification in the sludge-bed in 1-D laboratory-scale bioreactors was associated with increased richness in the underlying microbial community at species (OTU) level and improved overall performance.
dc.publisherFrontiers Media SA
dc.relation.ispartofFrontiers in Microbiology
dc.subject16s rrna gene
dc.subjectanaerobic digestion
dc.subjectegsb
dc.subjectillumina miseq
dc.subjectlaboratory-scale
dc.subjectfull-scale
dc.subjectindustrial wastewater
dc.subjectspecific methanogenic activity
dc.subjectanaerobic biological treatment
dc.subjectwaste-water treatment
dc.subjectbatch reactor
dc.subjectbacterial
dc.subjectegsb
dc.subjectsequences
dc.subjectdigestion
dc.subjectarchaeal
dc.subjectdiversity
dc.subjectdynamics
dc.titleBioreactor scalability: laboratory-scale bioreactor design influences performance, ecology, and community physiology in expanded granular sludge bed bioreactors
dc.typeArticle
dc.identifier.doi10.3389/fmicb.2017.00664
dc.local.publishedsourcehttp://journal.frontiersin.org/article/10.3389/fmicb.2017.00664/pdf
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