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dc.contributor.authorChan, Jeffrey C. Y.
dc.contributor.authorBurugapalli, Krishna
dc.contributor.authorNaik, Hemantkumar
dc.contributor.authorKelly, John L.
dc.contributor.authorPandit, Abhay
dc.date.accessioned2018-09-20T16:03:00Z
dc.date.available2018-09-20T16:03:00Z
dc.date.issued2008-02-01
dc.identifier.citationChan, Jeffrey C. Y. Burugapalli, Krishna; Naik, Hemantkumar; Kelly, John L.; Pandit, Abhay (2008). Amine functionalization of cholecyst-derived extracellular matrix with generation 1 pamam dendrimer. Biomacromolecules 9 (2), 528-536
dc.identifier.issn1525-7797,1526-4602
dc.identifier.urihttp://hdl.handle.net/10379/10740
dc.description.abstractA method to functionalize cholecyst-derived extracellular matrix (CEM) with free amine groups was established in an attempt to improve its potential for tethering of bioactive molecules. CEM was incorporated with Generation-1 polyamidoamine (G1 PAMAM) dendrimer by using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-hydroxysuccinimide cross-linking system. The nature of incorporation of PAMAM dendrimer was evaluated using shrink temperature measurements, Fourier transform infrared (FTIR) assessment, ninhydrin assay, and swellability. The effects of PAMAM incorporation on mechanical and degradation properties of CEM were evaluated using a uniaxial mechanical test and collagenase degradation assay, respectively. Ninhydrin assay and FTIR assessment confirmed the presence of increasing free amine groups with increasing quantity of PAMAM in dendrimer-incorporated CEM (DENCEM) scaffolds. The amount of dendrimer used was found to be critical in controlling scaffold degradation, shrink temperature, and free amine content. Cell culture studies showed that fibroblasts seeded on DENCEM maintained their metabolic activity and ability to proliferate in vitro. In addition, fluorescence cell staining and scanning electron microscopy analysis of cell-seeded DENCEM showed preservation of normal fibroblast morphology and phenotype.
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofBiomacromolecules
dc.subjectcross-linked collagen
dc.subjectsmall-intestinal submucosa
dc.subjectdermal sheep collagen
dc.subjecttissue engineering applications
dc.subjectgrowth-factor delivery
dc.subjectbody-wall repair
dc.subjectabdominal-wall
dc.subjectregenerating capacity
dc.subjectbiological stability
dc.subjectphysical-properties
dc.titleAmine functionalization of cholecyst-derived extracellular matrix with generation 1 pamam dendrimer
dc.typeArticle
dc.identifier.doi10.1021/bm701055k
dc.local.publishedsourcehttp://bura.brunel.ac.uk/bitstream/2438/9113/2/Fulltext.pdf
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