A novel mouse model of in situ stenting
dc.contributor.author | Chamberlain, Janet | |
dc.contributor.author | Wheatcroft, Mark | |
dc.contributor.author | Arnold, Nadine | |
dc.contributor.author | Lupton, Henry | |
dc.contributor.author | Crossman, David C. | |
dc.contributor.author | Gunn, Julian | |
dc.contributor.author | Francis, Sheila | |
dc.date.accessioned | 2018-09-20T16:03:00Z | |
dc.date.available | 2018-09-20T16:03:00Z | |
dc.date.issued | 2009-07-25 | |
dc.identifier.citation | Chamberlain, Janet; Wheatcroft, Mark; Arnold, Nadine; Lupton, Henry; Crossman, David C. Gunn, Julian; Francis, Sheila (2009). A novel mouse model of in situ stenting. Cardiovascular Research 85 (1), 38-44 | |
dc.identifier.issn | 1755-3245,0008-6363 | |
dc.identifier.uri | http://hdl.handle.net/10379/10737 | |
dc.description.abstract | Aims Animal models of stenting are mostly limited to larger animals or involve substantial abdominal surgery in rodents. We aimed to develop a simple, direct model of murine stenting. Methods and results We designed a miniature, self-expanding, nitinol wire coil stent that was pre-loaded into a metal stent sheath. This was advanced into the abdominal aorta of the mouse, via femoral access, and the stent deployed. In-stent restenosis was investigated at 1, 3, 7, and 28 days post-stenting. The model was validated by investigation of neointima formation in mice deficient in signalling via the interleukin-1 receptor (IL-1R1), compared with other injury models. Ninety-two per cent of mice undergoing the procedure were successfully stented. All stented vessels were patent. Inflammatory cells were seen in the adventitia and around the stent strut up to 3 days post-stenting. At 3 days, an early neointima was present, building to a mature neointima at 28 days. In mice lacking IL-1R1, the neointima was 64% smaller than that in wild-type controls at the 28-day timepoint, in agreement with other models. Conclusion This is the first description of a successful model of murine in situ stenting, using a stent specifically tailored for use in small thin-walled arteries. The procedure can be undertaken by a single operator without the need for an advanced level of microsurgical skill and is reliable and reproducible. The utility of this model is demonstrated by a reduction in in-stent restenosis in IL-1R1-deficient mice. | |
dc.publisher | Oxford University Press (OUP) | |
dc.relation.ispartof | Cardiovascular Research | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | mouse stent model | |
dc.subject | balloon angioplasty | |
dc.subject | neointimal formation | |
dc.subject | porcine model | |
dc.subject | rat model | |
dc.subject | restenosis | |
dc.subject | injury | |
dc.subject | mice | |
dc.subject | atherosclerosis | |
dc.subject | interleukin-1 | |
dc.subject | implantation | |
dc.title | A novel mouse model of in situ stenting | |
dc.type | Article | |
dc.identifier.doi | 10.1093/cvr/cvp262 | |
dc.local.publishedsource | https://academic.oup.com/cardiovascres/article-pdf/85/1/38/17198766/cvp262.pdf | |
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