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    Characterization of human melanoma cell lines and melanocytes by proteome analysis

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    Date
    2011-09-01
    Author
    Caputo, Emilia
    Maiorana, Luigi
    Vasta, Valeria
    Pezzino, Franca M.
    Sunkara, Sudhir
    Wynne, Kieran
    Elia, Giuliano
    Marincola, Francesco M.
    McCubrey, James A.
    Libra, Massimo
    Travali, Salvatore
    Kane, Marian
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    Cited 18 times in Scopus (view citations)
    
    Recommended Citation
    Caputo, Emilia; Maiorana, Luigi; Vasta, Valeria; Pezzino, Franca M. Sunkara, Sudhir; Wynne, Kieran; Elia, Giuliano; Marincola, Francesco M.; McCubrey, James A.; Libra, Massimo; Travali, Salvatore; Kane, Marian (2011). Characterization of human melanoma cell lines and melanocytes by proteome analysis. Cell Cycle 10 (17), 2924-2936
    Published Version
    http://www.tandfonline.com/doi/pdf/10.4161/cc.10.17.17068?needAccess=true
    Abstract
    We have analyzed the proteomes of two human melanoma cell lines (A375 and 526), and of the human melanocytes, (FOM 78), by two-dimensional electrophoresis (2D-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Our comparative proteomic analysis revealed that six proteins were overexpressed in both melanoma cell lines as compared with melanocytes: galectin-1, inosine-5'-monophosphate dehydrogenase 2, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, protein DJ-1, cyclophilin A and cofilin-1. We show, for the first time, that only specific isoforms of these molecules are overexpressed in melanoma. Different protein profiles were also found between each individual melanoma cell line and the melanocytes. s-Methyl-5-thioadenosine phosphorylase, ubiquitin and ribosomal protein S27 a precursor, the basic form of protein DJ-1, annexin a1, proliferation associated protein 2g4, isoform alfa-enolase of alfa-enolase, protein disulfide-isomerase precursor and elongation factor 2 were more strongly expressed in A375 cells compared with melanocytes. In 526 cells, 60s acidic ribosomal protein p1 and calreticulin precursor were more highly expressed than in melanocytes. These molecular differences may help in better understanding melanoma development and its different responsiveness to therapies. The identified proteins could be exploited as biomarkers or therapeutic targets for melanoma.
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    http://hdl.handle.net/10379/10686
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