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dc.contributor.authorZwacka,
dc.date.accessioned2018-09-20T16:01:59Z
dc.date.available2018-09-20T16:01:59Z
dc.date.issued2009-04-06
dc.identifier.citationZwacka, (2009). The trail-receptor-1: trail-receptor-3 and -4 ratio is a predictor for trail sensitivity of cancer cells. Oncology Reports 21 (5), 1289-1295
dc.identifier.issn1021-335X
dc.identifier.urihttp://hdl.handle.net/10379/10591
dc.description.abstractThe tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis in many cancer cells. However, a significant proportion of tumours are TRAIL-resistant erecting a major hurdle for a successful TRAIL-based treatment regimen in the future. In this context, it would be a major advantage to be able to identify the tumours that respond to TRAIL. The existence of two apoptosis-inducing receptors (TRAIL-R1 and TRAIL-R2) and two receptors that cannot transmit an apoptotic signal and have an inhibitory function (TRAIL-R3 and TRAIL-R4) make TRAIL signalling complicated. We analysed the surface expression of all four membrane-bound TRAIL receptors in cancer cell lines of various origin and primary cancer and normal cells and found a good correlation between TRAIL-sensitivity and the expression of TRAIL-R1 alone, but an even better correlation when a ratio of TRAIL-R1/TRAIL-R3+TRAIL-R4 was analysed. Experimental overexpression of TRAIL-R1 alone or in combination with TRAIL-R4 in PANC-1 cells confirmed our correlation results. Similar to the surface expression-apoptosis correlation analysis we found a high correlation between TRAIL-sensitivity and the mRNA level ratio of TRAIL-R1/TRAIL-R3+TRAIL-R4. A value of <0.85 for the ratio predicted TRAIL resistance in both protein and RNA analysis. Hence, TRAIL receptor RNA expression analysis by real-time PCR might be a feasible approach to predict possible TRAIL-responses in individual tumour samples.
dc.publisherSpandidos Publications
dc.relation.ispartofOncology Reports
dc.subjectapoptosis
dc.subjecttumour necrosis factor-related apoptosis-inducing ligand
dc.subjectapoptosis-inducing ligand 2
dc.subjecttumour necrosis factor-related apoptosis-inducing ligand receptor
dc.subjectdecoy receptor
dc.subjectreal-time pcr
dc.subjectapoptosis-inducing ligand
dc.subjectnf-kappa-b
dc.subjectdecoy receptors
dc.subjectdeath receptor
dc.subjectosteosarcoma cells
dc.subjectcolon-carcinoma
dc.subjectleukemia cells
dc.subjectlung-cancer
dc.subjecttumor-cells
dc.subjectresistance
dc.titleThe trail-receptor-1: trail-receptor-3 and -4 ratio is a predictor for trail sensitivity of cancer cells
dc.typeArticle
dc.identifier.doi10.3892/or_00000353
dc.local.publishedsourcehttp://www.spandidos-publications.com/or/21/5/1289/download
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