dc.contributor.author | Bowes, T. | |
dc.contributor.author | Hanley, S. A. | |
dc.contributor.author | Liew, A. | |
dc.contributor.author | Eglon, M. | |
dc.contributor.author | Mashayekhi, K. | |
dc.contributor.author | O'Kennedy, R. | |
dc.contributor.author | Barry, F. | |
dc.contributor.author | Taylor, W. R. | |
dc.contributor.author | O'Brien, T. | |
dc.contributor.author | Griffin, M. D. | |
dc.contributor.author | Finlay, W. J. J. | |
dc.contributor.author | Greiser, U. | |
dc.date.accessioned | 2018-09-20T16:01:27Z | |
dc.date.available | 2018-09-20T16:01:27Z | |
dc.date.issued | 2011-05-18 | |
dc.identifier.citation | Bowes, T. Hanley, S. A.; Liew, A.; Eglon, M.; Mashayekhi, K.; O'Kennedy, R.; Barry, F.; Taylor, W. R.; O'Brien, T.; Griffin, M. D.; Finlay, W. J. J.; Greiser, U. (2011). Developing cell-specific antibodies to endothelial progenitor cells using avian immune phage display technology. Journal of Biomolecular Screening 16 (7), 744-754 | |
dc.identifier.issn | 1087-0571,1552-454X | |
dc.identifier.uri | http://hdl.handle.net/10379/10496 | |
dc.description.abstract | This study aims at generating immune chicken phage display libraries and single-chain antibodies (scFvs) specifically directed against cell surface markers of cultured peripheral blood mononuclear cells (PBMCs) that contain endothelial progenitor cells (EPCs). In contrast to previous approaches that use well-defined recombinant antigens attached to plastic surfaces that may alter the structure of the proteins, the authors describe a method that maintains the cell surface markers on live cells while providing the opportunity to rapidly screen entire libraries for antibodies that bind to unknown cell surface markers of progenitor/stem cells. Chickens immunized with live EPCs, consisting of a heterogeneous population of lymphocytes and monocytes, demonstrated a robust immune response. After three rounds of biopanning, the authors purified and characterized three unique scFvs called UG1-3. Codon-optimized recombinant UG1 (gUG-1) shows binding by flow cytometry to circulating CD14-positive cells in peripheral blood consistent with predominant expression of a target protein on monocyte subsets. The authors describe the successful use of immunization of chickens for the generation of scFvs against a heterogenous population of EPCs displaying unknown cell surface markers and demonstrate the strong potential of phage display technology in the development of reagents for the isolation and characterization of stem/progenitor cells. (Journal of Biomolecular Screening. 2011; 16: 744-754) | |
dc.publisher | SAGE Publications | |
dc.relation.ispartof | Journal of Biomolecular Screening | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | phage display | |
dc.subject | single-chain antibodies | |
dc.subject | endothelial progenitor cells | |
dc.subject | cell surface markers | |
dc.subject | vasculature | |
dc.subject | coronary-artery-disease | |
dc.subject | monoclonal-antibody | |
dc.subject | peripheral-blood | |
dc.subject | chicken genome | |
dc.subject | fragments | |
dc.subject | generation | |
dc.subject | angiogenesis | |
dc.subject | phenotype | |
dc.subject | libraries | |
dc.subject | system | |
dc.title | Developing cell-specific antibodies to endothelial progenitor cells using avian immune phage display technology | |
dc.type | Article | |
dc.identifier.doi | 10.1177/1087057111407067 | |
dc.local.publishedsource | http://journals.sagepub.com/doi/pdf/10.1177/1087057111407067 | |
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