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dc.contributor.authorBist, Itti
dc.contributor.authorSong, Boya
dc.contributor.authorMosa, Islam M.
dc.contributor.authorKeyes, Tia E.
dc.contributor.authorMartin, Aaron
dc.contributor.authorForster, Robert J.
dc.contributor.authorRusling, James F.
dc.date.accessioned2018-09-20T16:01:11Z
dc.date.available2018-09-20T16:01:11Z
dc.date.issued2016-03-25
dc.identifier.citationBist, Itti; Song, Boya; Mosa, Islam M. Keyes, Tia E.; Martin, Aaron; Forster, Robert J.; Rusling, James F. (2016). Electrochemiluminescent array to detect oxidative damage in ds-dna using [os(bpy)2(phen-benz-cooh)]2+/nafion/graphene films. ACS Sensors 1 (3), 272-278
dc.identifier.issn2379-3694,2379-3694
dc.identifier.urihttp://hdl.handle.net/10379/10449
dc.description.abstractReactive oxygen species (ROS) oxidize guanosines in DNA to form 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG), a biomarker for oxidative stress. Herein we describe a novel 64-microwell electrochemiluminescent (ECL) array enabling sensitive multiplexed detection of 8-oxodG in dsDNA without hydrolysis. Films of Nafion and reduced graphene oxide containing ECL dye [Os(bpy)(2)(phen-benz-COOH)](2+) (OsNG, {bpy= 2,2'-bipyridine and phen-benz-COOH = (4-(1,10-phenanthrolin-6-yl)benzoic acid)}) were assembled into microwells on a pyrolytic graphite wafer to detect 8-oxodG in oligonucleotides by electrochemiluminescence (ECL). DNA oxidation by Fenton's reagent or by ROS formation during redox cycles involving NADPH, Cu-II, and model metabolites was monitored. UPLC-MS/MS of oxidized DNA samples were used for calibration. Detection limit for the fluidic arrays was one 8-oxodG per 670 intact nucleobases, or 0.15%. The method is sensitive enough to evaluate DNA oxidation from biologically relevant ROS-generating reactions of Cull, NADPH, and model metabolites.
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofACS Sensors
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectelectrochemiluminescence
dc.subjectoxidative stress
dc.subjectDNA oxidation sensor
dc.subjectmicrowell array
dc.subject[os(bpy)(2)(phen-benz-cooh)](2+)
dc.subjecttandem mass-spectrometry
dc.subjectDNA-damage
dc.subjectliquid-chromatography
dc.subjectelectrogenerated chemiluminescence
dc.subjectelectron-transfer
dc.subjectstress
dc.subjectcancer
dc.subjectcarcinogenesis
dc.subjectmechanisms
dc.subjectbiomarker
dc.titleElectrochemiluminescent array to detect oxidative damage in ds-dna using [os(bpy)2(phen-benz-cooh)]2+/nafion/graphene films
dc.typeArticle
dc.identifier.doi10.1021/acssensors.5b00189
dc.local.publishedsourcehttp://europepmc.org/articles/pmc4847550?pdf=render
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland